LOCUS       Exported                3519 bp ds-DNA     circular SYN 30-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ141B2.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 30, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pYPQ104_correct_ 3623 bp ds-DNA circular 02-MAR-2014 SOURCE 
            ORGANISM COMMENT COMMENT ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..161
                     /label=pYPQ104_correct_
                     /note="color: #ff8080"
     misc_feature    1..24
                     /note="M13-F1"
                     /note="color: #50ff50"
     primer_bind     17..34
                     /label=M13-fwd
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    36..148
                     /label=attL5
                     /note="color: #0080ff"
     promoter        182..507
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
                     /note="color: #0000ff; direction: RIGHT"
     modified_base   257
                     /note="G to c"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    443..452
                     /note="USE"
                     /note="color: #0080ff"
     TATA_signal     477..484
                     /note="TATA box"
                     /note="color: #00ff00"
     misc_feature    508
                     /note="first necletide transcript"
                     /note="color: #0080ff"
     misc_structure  509..540
                     /note="guide RNA cloning site"
                     /note="color: #0000ff; direction: RIGHT"
     stem_loop       541..683
                     /note="guide RNA scaffold 2.0"
                     /note="color: #0000ff; direction: BOTH"
     terminator      684..858
                     /label=AtU3 terminator
                     /note="AtU3 terminator"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    879..978
                     /label=attL2
                     /note="color: #0080ff"
     primer_bind     complement(995..1016)
                     /label=T7
                     /note="color: #8080ff; direction: LEFT"
     primer_bind     complement(1015..1035)
                     /label=M13-rev
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(1019..1035)
                     /label=M13-R
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    1113..1246
                     /label=Spn promoter
                     /note="color: #0080ff"
     misc_feature    1247..2257
                     /label=SpnR
                     /note="color: #0080ff"
     misc_feature    2324..2997
                     /label=pUC origin
                     /note="color: #0080ff"
ORIGIN
        1 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccca aataatgatt
       61 ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata
      121 atgccaactt tgtatacaaa agttgcccca tggcgttccc tctagataac gcaggatcct
      181 atttacttta aatttttctt atggctcagc ctgtgatgga taactgaatc aaacaaatgg
      241 cgtctgggtt taagaacatc tgttttggct atgttggacg aaacaagtga acttttagga
      301 tcaacttccg tttatatacg gagcttatat cgagcaataa gataagtggg ctttttatgt
      361 aatttaatgg gctatcgtcc atatattcac taatacccat gcccagtacc catgtatgcg
      421 tttcatataa gctcctaatt tctcccacat cgctcaaatc taaacaaatc ttgttgtata
      481 tataacactg agggagcacc attggtcaga gacgagatct agtctgagtc gaccgtctct
      541 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
      601 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      661 ggcaccgagt cggtgctttt ttttcccttt ccttttttct tttttttgcc ataaacttaa
      721 atttgtatat cgatcattgt agatattgaa aacctagaac aaaccaacat ccatgtgaat
      781 gtctttcatg actgatttag agataattct tgaattttgg aactagaatc tataatgagc
      841 ctaaattaaa acattgtgaa ctagtaaggg cgaattcgac ccagctttct tgtacaaagt
      901 tggcattata aaaaataatt gctcatcaat ttgttgcaac gaacaggtca ctatcagtca
      961 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     1021 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     1081 agataaaaat atatcatcat gcctcctctg gaccagccag gacagaaatg cctcgacttc
     1141 gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     1201 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     1261 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     1321 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     1381 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     1441 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
     1501 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     1561 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     1621 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     1681 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     1741 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     1801 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     1861 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     1921 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     1981 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     2041 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     2101 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     2161 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     2221 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     2281 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2461 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     2761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3001 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3061 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
     3241 tcgccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
     3301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     3361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     3421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
     3481 gcagttccct actctcgcgt taacgcttgc atggatgtt
//
