LOCUS       Exported                3358 bp ds-DNA     circular SYN 30-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ141C2.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 30, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pYPQ104_correct_ 3623 bp ds-DNA circular 02-MAR-2014 SOURCE 
            ORGANISM COMMENT COMMENT ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..161
                     /label=pYPQ104_correct_
                     /note="color: #000000"
     misc_feature    1..24
                     /note="M13-F1"
                     /note="color: #50ff50"
     primer_bind     17..34
                     /label=M13-fwd
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    36..148
                     /label=attL5
                     /note="color: #0000ff; direction: RIGHT"
     promoter        180..460
                     /label=OsU6-2 promoter
                     /note="OsU6-2 promoter"
                     /note="color: #0000ff; direction: RIGHT"
     TATA_signal     434..437
                     /note="TATA box"
                     /note="color: #00ff00"
     misc_structure  462..493
                     /note="guide RNA cloning site"
                     /note="color: #0080ff"
     stem_loop       494..636
                     /note="guide RNA scaffold 2.0"
                     /note="color: #00ff00"
     terminator      637..710
                     /label=OsU6-2 terminator
                     /note="OsU6-2 terminator"
                     /note="color: #000000"
     misc_feature    complement(688..710)
                     /label=AAGCTTATCGATACCCTCGACCT
                     /note="pYPQ13xC-R"
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    718..817
                     /label=attL2
                     /note="color: #0080ff"
     primer_bind     complement(834..855)
                     /label=T7
                     /note="color: #8080ff; direction: LEFT"
     primer_bind     complement(854..874)
                     /label=M13-rev
                     /note="color: #000000; direction: LEFT"
     misc_feature    complement(858..874)
                     /label=M13-R
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    952..1085
                     /label=Spn promoter
                     /note="color: #0080ff"
     misc_feature    1086..2096
                     /label=SpnR
                     /note="color: #0080ff"
     misc_feature    2163..2836
                     /label=pUC origin
                     /note="color: #0080ff"
ORIGIN
        1 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccca aataatgatt
       61 ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata
      121 atgccaactt tgtatacaaa agttgcccca tggcgttccc tctagataac gcaggatcca
      181 ctagtaacgg ccgccagtgt gctggaattg cccttggatc atgaaccaac ggcctggctg
      241 tatttggtgg ttgtgtaggg agatggggag aagaaaagcc cgattctctt cgctgtgatg
      301 ggctggatgc atgcggggga gcgggaggcc caagtacgtg cacggtgagc ggcccacagg
      361 gcgagtgtga gcgcgagagg cgggaggaac agtttagtac cacattgccc agctaactcg
      421 aacgcgacca acttataaac ccgcgcgctg tcgcttgtgt ggagacgaga tctagtctga
      481 gtcgaccgtc tctgttttag agctaggcca acatgaggat cacccatgtc tgcagggcct
      541 agcaagttaa aataaggcta gtccgttatc aacttggcca acatgaggat cacccatgtc
      601 tgcagggcca agtggcaccg agtcggtgct ttttttgtcc cttcgaaggg caattctgca
      661 gatatccatc acactggcgg ccgctcgagg tcgagggtat cgataagctt gaattcgacc
      721 cagctttctt gtacaaagtt ggcattataa aaaataattg ctcatcaatt tgttgcaacg
      781 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
      841 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      901 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctgg accagccagg
      961 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1021 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1081 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1141 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     1201 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1261 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     1321 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     1381 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     1441 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     1501 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     1561 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     1621 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     1681 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     1741 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     1801 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     1861 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     1921 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     1981 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2041 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     2101 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2161 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2221 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2281 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2341 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2401 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2461 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2521 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2581 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2641 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2701 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2761 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2821 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2881 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2941 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3001 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3061 caattaatac gcgtaccgct cgccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3121 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3181 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3241 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3301 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgcttgca tggatgtt
//
