LOCUS       Exported                3456 bp ds-DNA     circular SYN 30-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ141D2.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 30, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pYPQ104_correct_ 3623 bp ds-DNA circular 02-MAR-2014 SOURCE 
            ORGANISM COMMENT COMMENT ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..161
                     /label=pYPQ104_correct_
                     /note="color: #000000"
     misc_feature    1..24
                     /note="M13-F1"
                     /note="color: #50ff50"
     primer_bind     17..34
                     /label=M13-fwd
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    36..148
                     /label=attL5
                     /note="color: #0000ff; direction: RIGHT"
     promoter        182..558
                     /label=OsU3 promoter
                     /note="OsU3 promoter"
                     /note="color: #ff8080"
     promoter        182..204
                     /label=AAGGGATCTTTAAACATACGAAC
                     /note="pYPQ-13xD-F"
                     /note="color: #ff8080"
     misc_structure  560..591
                     /note="guide RNA cloning site"
                     /note="color: #0080ff"
     stem_loop       592..734
                     /note="guide RNA scaffold 2.0"
                     /note="color: #00ff00"
     terminator      735..808
                     /label=OsU6-2 terminator
                     /note="OsU6-2 terminator"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    complement(786..808)
                     /label=AAGCTTATCGATACCCTCGACCT
                     /note="pYPQ13xD-R"
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    816..915
                     /label=attL2
                     /note="color: #0080ff"
     primer_bind     complement(932..953)
                     /label=T7
                     /note="color: #8080ff; direction: LEFT"
     primer_bind     complement(952..972)
                     /label=M13-rev
                     /note="color: #ff8080; direction: LEFT"
     misc_feature    complement(956..972)
                     /label=M13-R
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    1050..1183
                     /label=Spn promoter
                     /note="color: #0080ff"
     misc_feature    1184..2194
                     /label=SpnR
                     /note="color: #0080ff"
     misc_feature    2261..2934
                     /label=pUC origin
                     /note="color: #0080ff"
ORIGIN
        1 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccca aataatgatt
       61 ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata
      121 atgccaactt tgtatacaaa agttgcccca tggcgttccc tctagataac gcaggatcct
      181 aaagggatct ttaaacatac gaacagatca cttaaagttc ttctgaagca acttaaagtt
      241 atcaggcatg catggatctt ggaggaatca gatgtgcagt cagggaccat agcacaggac
      301 aggcgtcttc tactggtgct accagcaaat gctggaagcc gggaacactg ggtacgttgg
      361 aaaccacgtg atgtggagta agataaactg taggagaaaa gcatttcgta gtgggccatg
      421 aagcctttca ggacatgtat tgcagtatgg gccggcccat tacgcaattg gacgacaaca
      481 aagactagta ttagtaccac ctcggctatc cacatagatc aaagctggtt taaaagagtt
      541 gtgcagatga tccgtggcag agacgagatc tagtctgagt cgaccgtctc tgttttagag
      601 ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa taaggctagt
      661 ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag tggcaccgag
      721 tcggtgcttt ttttgtccct tcgaagggca attctgcaga tatccatcac actggcggcc
      781 gctcgaggtc gagggtatcg ataagcttga attcgaccca gctttcttgt acaaagttgg
      841 cattataaaa aataattgct catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
      901 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
      961 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     1021 taaaaatata tcatcatgcc tcctctggac cagccaggac agaaatgcct cgacttcgct
     1081 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     1141 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     1201 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     1261 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     1321 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     1381 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     1441 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     1501 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     1561 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     1621 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     1681 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     1741 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     1801 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     1861 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     1921 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     1981 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     2041 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     2101 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     2161 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     2221 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2401 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2641 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     2701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3061 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctcg
     3181 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
     3241 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
     3301 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     3361 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
     3421 gttccctact ctcgcgttaa cgcttgcatg gatgtt
//
