LOCUS Exported 8897 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS AIRAP 9: PSMD2.GST.V2.pcDNA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8897) AUTHORS Stanhill A, Haynes CM, Zhang Y, Min G, Steele MC, Kalinina J, Martinez E, Pickart CM, Kong XP, Ron D TITLE An arsenite-inducible 19S regulatory particle-associated protein adapts proteasomes to proteotoxicity. JOURNAL Mol Cell. 2006 Sep 15. 23(6):875-85. PUBMED 16973439 REFERENCE 2 (bases 1 to 8897) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8897 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3728..4381 /codon_start=1 /product="glutathione S-transferase from Schistosoma japonicum" /label=GST /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK" primer_bind 4339..4361 /label=pGEX 5' /note="pGEX vectors, Glutathione-S-transferase, forward primer" CDS 4388..4405 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" promoter complement(4449..4467) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4450..4467) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4487..4504) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4493..4717 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4763..5191 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4850..4869) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5060..5081 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5200..5220) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5205..5535 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5386..5521 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5448..5467 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5602..6396 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5656..5675) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6266..6285 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6570..6691 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6607..6626) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6661..6680 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6740..6756) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6740..6756) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6753..6775) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6764..6780 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6788..6818) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6833..6854 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6971..6988) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7142..7730) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7222..7241) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7901..8761) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8524..8543 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8762..8866) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcggcacga gcgggccggc 961 agtggcggcg gagatggagg agggaggccg ggacaaggcg ccggtgcagc cccagcagtc 1021 tccagcggcg gcccccggcg gcacggacga gaagccgagc ggcaaggagc ggcgggatgc 1081 cggggacaag gacaaagaac aggagctgtc tgaagaggat aaacagcttc aagatgaact 1141 ggagatgctc gtggaacgac taggggagaa ggatacatcc ctgtatcgac cagcgctgga 1201 ggaattgcga aggcagattc gttcttctac aacttccatg acttcagtgc ccaagcctct 1261 caaatttctg cgtccacact atggcaaact gaaggaaatc tatgagaaca tggcccctgg 1321 ggagaataag cgttttgctg ctgacatcat ctccgttttg gccatgacca tgagtgggga 1381 gcgtgagtgc ctcaagtatc ggctagtggg ctcccaggag gaattggcat catggggtca 1441 tgagtatgtc aggcatctgg caggagaagt ggctaaggag tggcaggagc tggatgacgc 1501 agagaaggtc cagcgggagc ctctgctcac tctggtgaag gaaatcgtcc cctataacat 1561 ggcccacaat gcagagcatg aggcttgcga cctgcttatg gaaattgagc aggtggacat 1621 gctggagaag gacattgatg aaaatgcata tgcaaaggtc tgcctttatc tcaccagttg 1681 tgtgaattac gtgcctgagc ctgagaactc agccctactg cgttgtgccc tgggtgtgtt 1741 ccgaaagttt agccgcttcc ctgaagctct gagattggca ttgatgctca atgacatgga 1801 gttggtagaa gacatcttca cctcctgcaa ggatgtggta gtacagaaac agatggcatt 1861 catgctaggc cggcatgggg tgttcctgga gctgagtgaa gatgtcgagg agtatgagga 1921 cctgacagag atcatgtcca atgtacagct caacagcaac ttcttggcct tagctcggga 1981 gctggacatc atggagccca aggtgcctga tgacatctac aaaacccacc tagagaacaa 2041 caggtttggg ggcagtggct ctcaggtgga ctctgcccgc atgaacctgg cctcctcttt 2101 tgtgaatggc tttgtgaatg cagcttttgg ccaagacaag ctgctaacag atgatggcaa 2161 caaatggctt tacaagaaca aggaccacgg aatgttgagt gcagctgcat ctcttgggat 2221 gattctgctg tgggatgtgg atggtggcct cacccagatt gacaagtacc tgtactcctc 2281 tgaggactac attaagtcag gagctcttct tgcctgtggc atagtgaact ctggggtccg 2341 gaatgagtgt gaccctgctc tggcactgct ctcagactat gttctccaca acagcaacac 2401 catgagactt ggttccatct ttgggctagg cttggcttat gctggctcaa atcgtgaaga 2461 tgtcctaaca ctgctgctgc ctgtgatggg agattcaaag tccagcatgg aggtggcagg 2521 tgtcacagct ttagcctgtg gaatgatagc agtagggtcc tgcaatggag atgtaacttc 2581 cactatcctt cagaccatca tggagaagtc agagactgag ctcaaggata cttatgctcg 2641 ttggcttcct cttggactgg gtctcaacca cctggggaag ggtgaggcca tcgaggcaat 2701 cctggctgca ctggaggttg tgtcagagcc attccgcagt tttgccaaca cactggtgga 2761 tgtgtgtgca tatgcaggct ctgggaatgt gctgaaggtg cagcagctgc tccacatttg 2821 tagcgaacac tttgactcca aagagaagga ggaagacaaa gacaagaagg aaaagaaaga 2881 caaggacaag aaggaagccc ctgctgacat gggagcacat cagggagtgg ctgttctggg 2941 gattgccctt attgctatgg gggaggagat tggtgcagag atggcattac gaacctttgg 3001 ccacttgctg agatatgggg agcctacact ccggagggct gtacctttag cactggccct 3061 catctctgtt tcaaatccac gactcaacat cctggatacc ctaagcaaat tctctcatga 3121 tgctgatcca gaagtttcct ataactccat ttttgccatg ggcatggtgg gcagtggtac 3181 caataatgcc cgtctggctg caatgctgcg ccagttagct caatatcatg ccaaggaccc 3241 aaacaacctc ttcatggtgc gcttggcaca gggcctgaca catttaggga agggcaccct 3301 taccctctgc ccctaccaca gcgaccggca gcttatgagc caggtggccg tggctggact 3361 gctcactgtg cttgtctctt tcctggatgt tcgaaacatt attctaggca aatcacacta 3421 tgtattgtat gggctggtgg ctgccatgca gccccgaatg ctggttacgt ttgatgagga 3481 gctgcggcca ttgccagtgt ctgtccgtgt gggccaggca gtggatgtgg tgggccaggc 3541 tggcaagccg aagactatca cagggttcca gacgcataca accccagtgt tgttggccca 3601 cggggaacgg gcagaattgg ccactgagga gtttcttcct gttaccccca ttctggaagg 3661 ttttgttatc cttcggaaga accccaatta tgatctctcc catccatcac actggcggcc 3721 gctcgagatg tcccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg 3781 acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg 3841 tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta 3901 tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa 3961 gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc 4021 ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct 4081 caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg 4141 tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc 4201 tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg 4261 ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta 4321 tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa 4381 atcggatctg gttccgcgtg gatccccggg aatttccggt ggtggtggtg gaatctagag 4441 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct 4501 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 4561 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 4621 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 4681 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 4741 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 4801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 4861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc 4921 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 4981 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 5041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 5101 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat 5161 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 5221 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag 5281 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 5341 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 5401 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 5461 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 5521 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag 5581 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 5641 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 5701 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 5761 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 5821 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 5881 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 5941 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 6001 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 6061 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 6121 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 6181 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 6241 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 6301 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 6361 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 6421 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 6481 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 6541 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca 6601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 6661 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct 6721 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6781 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6841 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6901 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 6961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7321 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7561 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 7621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7921 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 7981 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 8041 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8101 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8161 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8221 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8281 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8341 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8401 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8461 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8521 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8581 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8641 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8701 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8761 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8821 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8881 aagtgccacc tgacgtc //