LOCUS       Exported                4004 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  aTc-inducible SCRIBE_lacZ(ON).
ACCESSION   .
VERSION     .
KEYWORDS    pFF838
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Farzadfard F, Lu TK
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations.
  JOURNAL   Science. 2014 Nov 14;346(6211):1256272. doi: 
            10.1126/science.1256272.
  PUBMED    25395541
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..80
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1312..2097
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     terminator      2115..2201
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2236..2253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2370..2915)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2450..2469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3029..3123
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3144..3803)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3718..3737
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3804..3906)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3972..3990
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 atcagcagga cgcactgacc ttaattaaat gcgcaccctt agcgagaggt ttatcattaa
      121 ggtcaacctc tggatgttgt ttcggcatcc tgcattgaat ctgagttact gtctgttttc
      181 ctgaattcag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      241 tgtgctgcaa ggcgattaag ttgggtgaat tcaggaaacc cgttttttct gacgtaaggg
      301 tgcgcaactt tcatgaaatc cgctgaatat ttgaacactt ttagattgag aaatctcggc
      361 ctacctgtca tgaacaattt gcatgacatg tctaaggcga ctcgcatatc tgttgaaaca
      421 cttcggttgt taatctatac agctgatttt cgctatagga tctacactgt agaaaagaaa
      481 ggcccagaga agagaatgag aaccatttac caaccttctc gagaacttaa agccttacaa
      541 ggatgggttc tacgtaacat tttagataaa ctgtcgtcat ctcctttttc tattggattt
      601 gaaaagcacc aatctatttt gaataatgct accccgcata ttggggcaaa ctttatactg
      661 aatattgatt tggaggattt tttcccaagt ttaactgcta acaaagtttt tggagtgttc
      721 cattctcttg gttataatcg actaatatct tcagttttga caaaaatatg ttgttataaa
      781 aatctgctac cacaaggtgc tccatcatca cctaaattag ctaatctaat atgttctaaa
      841 cttgattatc gtattcaggg ttatgcaggt agtcggggct tgatatatac gagatatgcc
      901 gatgacctca ccttatctgc acagtctatg aaaaaggttg ttaaagcacg tgatttttta
      961 ttttctataa tcccaagtga aggattggtt attaactcaa aaaaaacttg tattagtggg
     1021 cctcgtagtc agaggaaagt tacaggttta gttatttcac aagagaaagt tgggataggt
     1081 agagaaaaat ataaagaaat tagagcaaag atacatcata tattttgcgg taagtcttct
     1141 gagatagaac acgttagggg atggttgtca tttattttaa gtgtggattc aaaaagccat
     1201 aggagattaa taacttatat tagcaaatta gaaaaaaaat atggaaagaa ccctttaaat
     1261 aaagcgaaga cctaaggatc cggttgatat tgattcagag gtataaaacg aatgagtact
     1321 gcactcgcaa cgctggctgg gaagctggct gaacgtgtcg gcatggattc tgtcgaccca
     1381 caggaactga tcaccactct tcgccagacg gcatttaaag gtgatgccag cgatgcgcag
     1441 ttcatcgcat tactgatcgt tgccaaccag tacggcctta atccgtggac gaaagaaatt
     1501 tacgcctttc ctgataagca gaatggcatc gttccggtgg tgggcgttga tggctggtcc
     1561 cgcatcatca atgaaaacca gcagtttgat ggcatggact ttgagcagga caatgaatcc
     1621 tgtacatgcc ggatttaccg caaggaccgt aatcatccga tctgcgttac cgaatggatg
     1681 gatgaatgcc gccgcgaacc attcaaaact cgcgaaggca gagaaatcac ggggccgtgg
     1741 cagtcgcatc ccaaacggat gttacgtcat aaagccatga ttcagtgtgc ccgtctggcc
     1801 ttcggatttg ctggtatcta tgacaaggat gaagccgagc gcattgtcga aaatactgca
     1861 tacactgcag aacgtcagcc ggaacgcgac atcactccgg ttaacgatga aaccatgcag
     1921 gagattaaca ctctgctgat cgccctggat aaaacatggg atgacgactt attgccgctc
     1981 tgttcccaga tatttcgccg cgacattcgt gcatcgtcag aactgacaca ggccgaagca
     2041 gtaaaagctc ttggattcct gaaacagaaa gccgcagagc agaaggtggc agcatgaacg
     2101 cgtgctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2161 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagacctagg
     2221 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     2281 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     2341 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     2401 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     2461 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     2521 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     2581 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     2641 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
     2701 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     2761 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     2821 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
     2881 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     2941 aatattacta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     3001 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     3061 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     3121 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     3181 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
     3241 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     3301 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     3361 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     3421 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     3481 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     3541 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
     3601 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     3661 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     3721 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     3781 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     3841 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     3901 cgatcaacgt ctcattttcg ccagatatcg acgtctaaga aaccattatt atcatgacat
     3961 taacctataa aaataggcgt atcacgaggc cctttcgtct tcac
//
