LOCUS Exported 13122 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.. ACCESSION . VERSION . KEYWORDS pH7WG2B-OsMIR390-B/c SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13122) AUTHORS Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, Carrington JC TITLE Highly Specific Gene Silencing in a Monocot Species by Artificial MicroRNAs Derived From Chimeric MIRNA Precursors. JOURNAL Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835. PUBMED 25809382 REFERENCE 2 (bases 1 to 13122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13122 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 115..139 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" protein_bind 1942..1966 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 2051..2081 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 2135..2794 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(2201..2220) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 3136..3441 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 3320..3339 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(3545..3569) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5231..6256 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" primer_bind complement(6271..6287) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6271..6287) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6284..6306) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6295..6311 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6319..6349) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6364..6385 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 6702..6726 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 7254..8045 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 8289..8877 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8778..8797 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9031..9048 /label=L4440 /note="L4440 vector, forward primer" misc_feature 9063..9203 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(9129..9148) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9289..9311 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 9547..9741 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(9807..10874) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" CDS complement(11308..11937) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" ORIGIN 1 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac 61 cttttcacgc ccttttaaat atccgttatt ctaataaacg ctcttttctc ttaggtttac 121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg 181 atccaagctc aagctaagct tattcgggtc aaggcggaag ccagcgcgcc accccacgtc 241 agcaaatacg gaggcgcggg gttgacggcg tcacccggtc ctaacggcga ccaacaaacc 301 agccagaaga aattacagta aaaaaaaagt aaattgcact ttgatccacc ttttattacc 361 taagtctcaa tttggatcac ccttaaacct atcttttcaa tttgggccgg gttgtggttt 421 ggactaccat gaacaacttt tcgtcatgtc taacttccct ttcagcaaac atatgaacca 481 tatatagagg agatcggccg tatactagag ctgatgtgtt taaggtcgtt gattgcacga 541 gaaaaaaaaa tccaaatcgc aacaatagca aatttatctg gttcaaagtg aaaagatatg 601 tttaaaggta gtccaaagta aaacttatag ataataaaat gtggtccaaa gcgtaattca 661 ctcaaaaaaa atcaacgaga cgtgtaccaa acggagacaa acggcatctt ctcgaaattt 721 cccaaccgct cgctcgcccg cctcgtcttc ccggaaaccg cggtggtttc agcgtggcgg 781 attctccaag cagacggaga cgtcacggca cgggactcct cccaccaccc aaccgccata 841 aataccagcc ccctcatctc ctctcctcgc atcagctcca cccccgaaaa atttctcccc 901 aatctcgcga ggctctcgtc gtcgaatcga atcctctcgc gtcctcaagg tacgctgctt 961 ctcctctcct cgcttcgttt cgattcgatt tcggacgggt gaggttgttt tgttgctaga 1021 tccgattggt ggttagggtt gtcgatgtga ttatcgtgag atgtttaggg gttgtagatc 1081 tgatggttgt gatttgggca cggttggttc gataggtgga atcgtggtta ggttttggga 1141 ttggatgttg gttctgatga ttggggggaa tttttacggt tagatgaatt gttggatgat 1201 tcgattgggg aaatcggtgt agatctgttg gggaattgtg gaactagtca tgcctgagtg 1261 attggtgcga tttgtagcgt gttccatctt gtaggccttg ttgcgagcat gttcagatct 1321 actgttccgc tcttgattga gttattggtg cggttggtgc aaacacaggc tttaatatgt 1381 tatatctgtt ttgtgtttga tgtagatctg tagggtagtt cttcttagac atggttcaat 1441 tatgtagctt gtgcgtttcg atttgatttc atatgttcac agattagata atgatgaact 1501 cttttaatta attgtcaatg gtaaatagga agtcttgtcg ctatatctgt cataatgatc 1561 tcatgttact atctgccagt aatttatgct aagaactata ttagaatatc atgttacaat 1621 ctgtagtaat atcatgttac aatctgtagt tcatctatat aatctattgt ggtaatttct 1681 ttttactatc tgtgtgaaga ttattgccac tagttcattc tacttatttc tgaagttcag 1741 gatacgtgtg ctgttactac ctatctgaat acatgtgtga tgtgcctgtt actatctttt 1801 tgaatacatg tatgttctgt tggaatatgt ttgctgtttg atccgttgtt gtgtccttaa 1861 tcttgtgcta gttcttaccc tatctgtttg gtgattattt cttgcagatt cagatcgggc 1921 ccaagcttga ctagtgatat cacaagtttg tacaaaaaag caggctccgc ggccgccccc 1981 ttcaccgagc tcgagatgtt ttgaggaagg gtatggaaca atccttgaga gaccattagg 2041 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga 2101 gccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac 2161 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 2221 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa 2281 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc 2341 tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 2401 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 2461 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 2521 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 2581 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 2641 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 2701 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca 2761 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggagccggct tactaaaagc 2821 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat 2881 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt 2941 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa 3001 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc 3061 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga 3121 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 3181 ctgtttgtgg atgtacagag tgatattatt gacacgcccg gccgacggat ggtgatcccc 3241 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 3301 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtttccgtt 3361 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 3421 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcacctcga 3481 cggtctcaca tggtttgttc ttaccacacg accaattaaa tcgagctcaa gggtgggcgc 3541 gccgacccag ctttcttgta caaagtggtg atatcccgcg gccatgctag agtccgcaaa 3601 aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga 3661 gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat 3721 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt 3781 cctaaaacca aaatccagtg acctgcaggc atgcgacgtc gggccctcta gaggatcccc 3841 gggtaccgtg cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat ctctgtcgct 3901 gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc tgtcggcatc 3961 cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc tcctcctcct 4021 ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct ttcccttcct 4081 cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct cgtgttgttc 4141 ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac ctccgcttca 4201 aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc ggcgttccgg 4261 tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt 4321 gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg 4381 attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca 4441 gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc 4501 ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg 4561 tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt agaaatctgt 4621 ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca 4681 tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat 4741 gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg 4801 tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct 4861 ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt 4921 taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat 4981 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct 5041 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca 5101 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg 5161 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtcgactc 5221 tagaggatcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 5281 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 5341 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 5401 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 5461 gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc gtgcacaggg 5521 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac cggtcgcgga 5581 ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 5641 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 5701 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 5761 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 5821 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 5881 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 5941 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 6001 aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 6061 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 6121 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 6181 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 6241 gagggcaaag aaataggaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 6301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6361 ctaatgagtg agctaactca cattacttaa gattgaatcc tgttgccggt cttgcgatga 6421 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga 6481 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga 6541 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt 6601 tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca gtacattaaa 6661 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc 6721 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat 6781 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact 6841 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac 6901 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg 6961 atcaattcgg gcacgaaccc agtggacata agcctcgttc ggttcgtaag ctgtaatgca 7021 agtagcgtaa ctgccgtcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa 7081 cggcgcagtg gcggttttca tggcttcttg ttatgacatg tttttttggg gtacagtcta 7141 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 7201 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg 7261 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc 7321 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga 7381 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc 7441 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc 7501 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag 7561 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg 7621 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg 7681 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat 7741 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg 7801 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg 7861 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg 7921 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg 7981 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca 8041 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgccggcgt taactcaagc 8101 gattagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt 8161 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc 8221 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 8281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 8341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 8401 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 8461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 8521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 8581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 8641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 8701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 8761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 8821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 8881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 8941 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 9001 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 9061 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 9121 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 9181 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 9241 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 9301 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg 9361 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt 9421 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt 9481 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca 9541 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag 9601 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca 9661 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc 9721 ccaatgtacg tgctatccac aggaaagaga acttttcgac ctttttcccc tgctagggca 9781 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg 9841 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac 9901 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct 9961 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct 10021 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa 10081 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa 10141 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag 10201 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc 10261 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct 10321 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg 10381 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga 10441 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct 10501 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg 10561 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc 10621 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta 10681 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc 10741 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc 10801 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg 10861 gatggtttgc gaccgtcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg 10921 ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca 10981 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa 11041 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag 11101 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat 11161 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca 11221 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt 11281 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag 11341 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc 11401 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc 11461 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga 11521 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc 11581 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa 11641 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac 11701 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc 11761 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg 11821 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt 11881 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg 11941 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc 12001 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg 12061 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc 12121 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc 12181 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg 12241 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt 12301 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa 12361 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc 12421 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc 12481 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga 12541 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg 12601 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct 12661 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg 12721 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt 12781 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt 12841 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc 12901 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact 12961 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc 13021 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga 13081 agccgactgc actatagcag cggaggggtt ggatcaaagt ac //