LOCUS Exported 6845 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS PERK 3: PERK.dC.9E10.pCDNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6845) TITLE PERK constructs REFERENCE 2 (bases 1 to 6845) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6845 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 471..490 /label=pBR322ori-F /note="pBR322 origin, forward primer" rep_origin complement(603..1061) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(740..761) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 955..974 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind complement(1186..1204) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1272..1376 /gene="bla" /label=AmpR promoter CDS 1377..2237 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1595..1614) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" enhancer 2310..2689 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2690..2893 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2844..2864 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2938..2957 /label=T7 /note="T7 promoter, forward primer" promoter 2938..2956 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 5057..5086 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" promoter complement(5117..5135) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(5118..5135) /label=SP6 /note="SP6 promoter, forward primer" intron 5219..5284 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 5414..5434 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 5706..5840 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5743..5762) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5797..5816 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6686..6821 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6748..6767 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" ORIGIN 1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct 601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga 721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc 841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga 1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta 1141 aagggatttt agacaggaac ggtacgccag ctggattacc aaagggcctc gtgatacgcc 1201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 1261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 1321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 1381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 1441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 1501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 1561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg 1621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 1681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 1741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 1801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 1861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 1921 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 1981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 2041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 2101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 2161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 2221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 2281 aacttcattt ttaatttctc tagcgcgttg acattgatta ttgactagtt attaatagta 2341 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 2401 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 2461 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt 2521 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 2581 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 2641 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 2701 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 2761 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 2821 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 2881 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa 2941 tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc 3001 gccagtgtgc tggaattcgg cacgagcgat gtctgcacaa ggctgtcact caggtggcag 3061 tggctgacac gtggccgggc agctctgctg ctgcggcgcg aagtcgagag gcggcggggt 3121 ccgtggcgcg cgctcgcatt gctccgaggc tccgagcggc gatacgggcg ggcgccgacg 3181 gcagggtctc catgcccgcg cgtggggcgg gccgctgatg gagcgcgcca cccggcccgg 3241 gccgcgcgcg ctgctgctgc ttctgttcct gctgctgggc tgcgcggcgg ggatctctgc 3301 ggtcgcgccc gcccgcagtt tgcttgctcc cgcgtcggag acagtgtttg gcttaggggc 3361 agcggccgcc ccgacttcgg ccgcgcgggt gcctgcggtg gcaacggccg aagtgaccgt 3421 ggaggacgcc gaggcattgc cggctgccgc tggcgaaccg gagtcacgcg cgacggagcc 3481 cgatgacgac gtggaactgc ggcctcgcgg caggtccttg gtaatcatca gcactttaga 3541 tggacgaatc gctgcactgg atgccgagaa tgatgggaaa aagcagtggg atttggacgt 3601 ggggtctggt tccttggttt catctagcct cagcaagcca gaggtgtttg ggaacaagat 3661 gatcatcccc tccctggatg gagacctctt ccagtgggac cgggaccgag agagcatgga 3721 ggccgtcccc ttcacggtgg agtccctgct cgaatcttcc tacaagtttg gagatgatgt 3781 tgttctggtt ggagggaaat ctctgattac atacggactc agtgcttaca gtggaaagct 3841 gaggtatatc tgttctgcct tgggatgtcg ccgatgggat agtgatgaaa tggaagaaga 3901 ggaagacatc ttgcttctgc agcgtacgca gaagactgtg cgagctgtcg ggcctcgaag 3961 cggcagtgag aagtggaatt tcagtgttgg ccactttgaa cttcggtata ttccagacat 4021 ggaaactaga gccggattca ttgaaagcac ctttaaaccg ggtggaaaca aagaagactc 4081 taaaattatt tcagatgtgg aagaacaaga agccaccatg ctggacacag tgataaaagt 4141 ttccgttgct gattggaagg tcatggcgtt tagtaggaag ggaggccgcc tggaatggga 4201 gtaccagttt tgtactccca tcgcgtccgc ctggctggtg agggatggca aggtcatccc 4261 catcagcctg tttgatgata caagttacac agccagcgaa gaagccttgg gagacgaaga 4321 agacattgta gaggctgctc ggggagccac agagaacagc gtgtacttag ggatgtacag 4381 aggccagctg tacctgcagt cgtccgtcag ggtctcagaa aagttcccta caagcccaaa 4441 ggccttggag tctgtaaatg gcgaaaatgc aattattcct ctgccgacga tcaaatggaa 4501 gcccttaatc cattctcctt ctaggactcc tgtcttggtt gggtctgatg aatttgacaa 4561 atgtctaagt aatgataagt attcccacga agaatacagt aatggtgcac tttcaatcct 4621 ccagtatcca tacgataacg gttactatct gccatactac aagagagaaa ggaataagcg 4681 gagcacgcag atcacagtca ggttcctgga cagcccccac tacagcaaga acatccgcaa 4741 gaaggaccct atcctcctgc tgcactggtg gaaggagata ttcgggacga tcctgctttg 4801 catcgtagcc acgaccttca tcgtgcgcag gcttttccat cctcagcccc acaggcagcg 4861 gaaggagtct gaaactcagt gccagactga aagtaaatac gactccgtga gtgccgatgt 4921 cagtgacaac agctggaatg acatgaagta ctcaggatac gggaaaacgg ttctgagaca 4981 gcggtcccgc tccatgagtt catctggaac aaaacattcc agacagccca gctgctcgta 5041 cacgccactg cctggcgagc agaagctgat ctctgaggag gatctgtgac tcgagcatgc 5101 atctagaggg ccctattcta tagtgtcacc taaatgctag aggatctttg tgaaggaacc 5161 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt 5221 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt 5281 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga 5341 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca 5401 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga 5461 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat 5521 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat 5581 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg 5641 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 5701 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5761 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 5821 tatcttatca tgtctggatc atcccgccat ggtatcaacg ccatatttct atttacagta 5881 gggacctctt cgttgtgtag gtaccgctgt attcctaggg aaatagtaga ggcaccttga 5941 actgtctgca tcagccatat agcccccgct gttcgactta caaacacagg cacagtactg 6001 acaaacccat acacctcctc tgaaataccc atagttgcta gggctgtctc cgaactcatt 6061 acaccctcca aagtcagagc tgtaatttcg ccatcaaggg cagcgagggc ttctccagat 6121 aaaatagctt ctgccgagag tcccgtaagg gtagacactt cagctaatcc ctcgatgagg 6181 tctactagaa tagtcagtgc ggctcccatt ttgaaaattc acttacttga tcagcttcag 6241 aagatggcgg agggcctcca acacagtaat tttcctcccg actcttaaaa tagaaaatgt 6301 caagtcagtt aagcaggaag tggactaact gacgcagctg gccgtgcgac atcctctttt 6361 aattagttgc taggcaacgc cctccagagg gcgtgtggtt ttgcaagagg aagcaaaagc 6421 ctctccaccc aggcctagaa tgtttccacc caatcattac tatgacaaca gctgtttttt 6481 ttagtattaa gcagaggccg gggacccctg ggcccgctta ctctggagaa aaagaagaga 6541 ggcattgtag aggcttccag aggcaacttg tcaaaacagg actgcttcta tttctgtcac 6601 actgtctggc cctgtcacaa ggtccagcac ctccataccc cctttaataa gcagtttggg 6661 aacgggtgcg ggtcttactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 6721 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6781 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 6841 aattc //