LOCUS Exported 9677 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Const. Halotag-GFP-Mito + mCherry-DHFR. ACCESSION . VERSION . KEYWORDS pERB217 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9677) AUTHORS Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM TITLE Localized light-induced protein dimerization in living cells using a photocaged dimerizer. JOURNAL Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475. PUBMED 25400104 REFERENCE 2 (bases 1 to 9677) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9677 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 52..100 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" protein_bind complement(312..345) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." CDS 350..949 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(350..369) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 846..866 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(1085..1102) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1091..1202 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" enhancer 1347..1650 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1652..1929 /label=chicken beta-actin promoter intron 1930..2946 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 2870..2892 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 2954..2973 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 3031..3040 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3037..3927 /codon_start=1 /product="modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives" /label=HaloTag(R) /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL IGSEIARWLSTLEISG" regulatory 3934..3943 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3940..4656 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3940..4656 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3985..4006) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4246..4265) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4593..4614 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4919..5492 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4942..5494 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5086..5103) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5313..5332 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5494..6201 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(5639..5657) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 5907..5926 /label=mCherry-F /note="mCherry, forward primer" CDS 6220..6693 /codon_start=1 /gene="folA" /product="E. coli dihydrofolate reductase" /label=ecDHFR /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR" CDS 6694..6723 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" primer_bind complement(6824..6843) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 6889..6944 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(6943..6962) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" protein_bind complement(7305..7338) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 7351..7472 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7388..7407) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7442..7461 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7491..7511) /label=T3 /note="T3 promoter, forward primer" promoter complement(7491..7509) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7530..7546) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7530..7546) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7543..7565) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7554..7570 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7578..7608) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7623..7644 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7761..7778) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7932..8520) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8012..8031) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8691..9551) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9314..9333 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9552..9656) /gene="bla" /label=AmpR promoter ORIGIN 1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca 301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta 361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc 421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga 481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca 961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc 1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga 1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta 1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact 1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt 1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc 1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc 1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg 1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg 1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc 2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg 2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg 2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc 2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc 2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg 2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc 2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt 2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg 2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg 2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc 2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc 2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg 2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg 2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc 2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc 2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg 3001 ctcgaccacc ggcgcgctgc agtacggcgc gccaccatgg cagaaatcgg tactggcttt 3061 ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt 3121 ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg 3181 cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga cctgatcggt 3241 atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt ccgcttcatg 3301 gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca cgactggggc 3361 tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg tattgcattt 3421 atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc ccgcgagacc 3481 ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca gaacgttttt 3541 atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat 3601 taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt cccaaacgag 3661 ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata catggactgg 3721 ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt tctgatccca 3781 ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc 3841 ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga gatcgcgcgc 3901 tggctgtcga cgctcgagat ttccggttac gtagccacca tggtgagcaa gggcgaggag 3961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 4021 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 4081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 4141 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 4201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 4261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 4321 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 4381 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 4441 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 4501 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 4561 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 4621 gccgggatca ctctcggcat ggacgagctg tacaagggtg gtgaatccgg tgatgcatcg 4681 ggaagtggaa gtggatctcg agctcaagct tcgaattcaa aactaattgc taaaagtgca 4741 gaagacgaaa aagcgaagga agaaccaggg aaccatagga tcgtaattct tgcaatgtta 4801 gctattggcg tgttctcttt aggggcgctt atcaaaatta ttcaattaag aaaaaataat 4861 taaggatcca ccggatctag ataactgatc tccggatgac gataagctta aggatccgcc 4921 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 4981 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 5041 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 5101 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 5161 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 5221 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 5281 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg 5341 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca 5401 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg 5461 acgtggtttt cctttgaaaa acacgatgat aatatggtga gcaagggcga ggaggataac 5521 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 5581 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 5641 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag 5701 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag 5761 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 5821 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg 5881 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag 5941 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg 6001 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag 6061 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac 6121 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc 6181 ggcatggacg agctgtataa ggccggcctg gagtctagaa tcagtctgat tgcggcgtta 6241 gcggtagatc gcgttatcgg catggaaaac gccatgccgt ggaacctgcc tgccgatctc 6301 gcctggttta aacgcaacac cttaaataaa cccgtgatta tgggccgcca tacctgggaa 6361 tcaatcggtc gtccgttgcc aggacgcaaa aatattatcc tcagcagtca accgggtacg 6421 gacgatcgcg taacgtgggt gaagtcggtg gatgaagcca tcgcggcgtg tggtgacgta 6481 ccagaaatca tggtgattgg cggcggtcgc gtttatgaac agttcttgcc aaaagcgcaa 6541 aaactgtatc tgacgcatat cgacgcagaa gtggaaggcg acacccattt cccggattac 6601 gagccggatg actgggaatc ggtattcagc gaattccacg atgctgatgc gcagaactct 6661 cacagctatt gctttgagat tctggagcgg cgggaacaaa aactcatctc agaagaggat 6721 ctttaattaa gcgctcgagg cggccgcctc ctcaggtgca ggctgcctat cagaaggtgg 6781 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca 6841 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat 6901 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg 6961 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg 7021 ccatatgctg gctgccatga acaaaggtgg ctataaagag gtcatcagta tatgaaacag 7081 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt 7141 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta 7201 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta 7261 tgaagatccc tcgacctgag cgtcgagcta gcatatggat ccatataact tcgtataatg 7321 tatgctatac gaagttatta cgataccgtc aacttgttta ttgcagctta taatggttac 7381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 7441 tgtggtttgt ccaaactcat caatgtatct taggtaccca gcttttgttc cctttagtga 7501 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 7561 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 7621 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 7681 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 7741 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 7801 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 7861 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 7921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 7981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 8041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 8101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8341 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 8401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 8461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 8521 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 8581 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 8641 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 8701 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 8761 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 8821 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 8881 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 8941 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 9001 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 9061 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9121 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9181 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9241 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9301 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 9361 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 9421 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 9481 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 9541 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 9601 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 9661 tttccccgaa aagtgcc //