LOCUS Exported 16295 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus. ACCESSION . VERSION . KEYWORDS Ai65(RCFL-tdT) targeting vector SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 16295) AUTHORS Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H TITLE Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. JOURNAL Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. PUBMED 25741722 REFERENCE 2 (bases 1 to 16295) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..16295 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 3..1085 /label=Rosa26 left arm /note="left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)" enhancer 1181..1484 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1486..1763 /label=chicken beta-actin promoter intron 1765..2782 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 2706..2728 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 2790..2809 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind 2864..2897 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" polyA_signal 2943..2991 /note="synthetic polyadenylation signal" polyA_signal 3000..3476 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3199..3218) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(3511..3530) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3555..3603 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" protein_bind 3763..3796 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" protein_bind complement(3811..3844) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 4011..4145 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 4265..4399 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 4519..4653 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind complement(4682..4715) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 4804..4813 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4810..6240 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" misc_feature 6319..6907 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6372..6392) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6790..6801) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6910..6926) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6911..6927) /label=pBluescriptKS /note="For pBluescript vector" primer_bind complement(6928..6945) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6934..7141 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" protein_bind 7149..7182 /label=attB /bound_moiety="phage phi-C31 integrase" /note="minimal attB site for the phi-C31 integrase (Groth et al., 2000)" promoter 7204..7703 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(7239..7262) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 7636..7655 /label=mPGK-F /note="Mouse PGK promoter, forward primer" promoter 7720..7767 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7795..8598 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7858..7877) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8468..8487 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 8608..9069 /label=PGK poly(A) signal /note="mouse phosphoglycerate kinase 1 polyadenylation signal" primer_bind complement(13447..13467) /label=T3 /note="T3 promoter, forward primer" promoter complement(13447..13465) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(13486..13502) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(13486..13502) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(13499..13521) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 13510..13526 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(13534..13564) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13579..13600 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(13717..13734) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13888..14476) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13968..13987) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14647..15507) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 15270..15289 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(15508..15612) /gene="bla" /label=AmpR promoter rep_origin complement(15638..16093) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(15775..15796) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 15987..16006 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 16220..16242 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 16234..16251 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 16235..16251 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 16261..16280 /label=T7 /note="T7 promoter, forward primer" promoter 16261..16279 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt 61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga 121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg 181 ggggcagcgg aggascgctt ccggccgacg tctcgtcgct gattggctty ttttcctccc 241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg 301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta 361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg 421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg 481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca 541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc 601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt 661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag 721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag 781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc 841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt 901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg 961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac 1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt 1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa 1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg 1621 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc 1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 1741 aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg 1801 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt 1861 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct 1921 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg 1981 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc 2041 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag 2101 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg 2161 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc 2221 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc 2281 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg 2341 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg 2401 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct 2461 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc 2521 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc 2581 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct 2641 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag 2701 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca 2761 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca 2821 ttttggcaaa gaattgattt gataccgcgg gcccgtattc gaagaagttc ctattctcta 2881 gaaagtatag gaacttccct cagcaccatg gctagcggca gcctcggagt ttgaatagat 2941 agaataaaat atctttattt tcattccatc tgtgtgttgg ttttttgtgt gagatctacg 3001 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag 3061 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct 3121 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa 3181 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc 3241 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcaggct cccgagttgt 3301 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 3361 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt 3421 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctgc 3481 ctcagctaat tgagtagggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg 3541 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg 3601 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc 3661 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg 3721 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccg tggaagttcc tattctctag 3781 aaagtatagg aacttcctcg agatttaaat ataacttcgt ataatgtatg ctatacgaag 3841 ttattcgcga tgaataaatg aaagcttgca gatctgcgac tctagaggat ctgcgactct 3901 agaggatcat aatcagccnt accacatttg tagaggtttt actngcttta aaaaacctcc 3961 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 4021 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 4081 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 4141 ggatctgcga ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc 4201 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 4261 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4321 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 4381 atcttatcat gtctggatct gcgactctag aggatcataa tcagccatac cacatttgta 4441 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 4501 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 4561 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4621 aaactcatca atgtatctta tcatgtctgg atccccatca agctgatccg gaacccttaa 4681 tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacct gcagcccaag 4741 ctagatcgaa ttcggccggc cttcacgtgt tgcacttaac gcgtggccgg ccttcacgat 4801 cccgccacca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg 4861 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 4921 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc 4981 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc 5041 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 5101 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc 5161 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg 5221 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg 5281 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag 5341 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac 5401 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc 5461 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc 5521 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc 5581 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag 5641 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg 5701 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag 5761 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag 5821 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag 5881 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc 5941 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac 6001 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg 6061 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac 6121 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 6181 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtaa 6241 gaattgtgtt gcacttaacg cgttaagtgc aacacgtgaa ggccggccct gcaggaattc 6301 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt 6361 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 6421 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6481 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6541 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6601 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6661 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 6721 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6781 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6841 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 6901 tccccgcatc gataccgtcg acctcgacct cgactgtgcc ttctagttgc cagccatctg 6961 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 7021 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 7081 gtggggtggg gcaggacagc aagggggagg attgggaaga caatggcagg catgctgggg 7141 aactagtggt gccagggcgt gcccttgggc tccccgggcg cggcggccgc atcgaattct 7201 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc 7261 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta 7321 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag 7381 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag 7441 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc 7501 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa 7561 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt 7621 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct 7681 cttcctcatc tccgggcctt tcgacctgca gcagcacgtg ttgacaatta atcatcggca 7741 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccgctag caccatggga 7801 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 7861 ttcggctatg actgggcaca acagacgatc ggctgctctg atgccgccgt gttccggctg 7921 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 7981 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 8041 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 8101 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 8161 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 8221 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 8281 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 8341 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 8401 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 8461 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 8521 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 8581 cttgacgagt tcttctgagg ggatccgctg taagtctgca gaaattgatg atctattaaa 8641 caataaagat gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag 8701 aacagagtac ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg 8761 attagataaa tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc 8821 atagttggat atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc 8881 actcatgatc tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca 8941 ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag 9001 atcagcagcc tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt 9061 ccatcagaaa gcttgcagaa gatctcccca actggggtaa cctttgagtt ctctcagttg 9121 ggggggcgcg ccctagaaga tgggcgggag tcttctgggc aggcttaaag gctaacctgg 9181 tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa aattggaggg acaagacttc 9241 ccacagattt tcggttttgt cgggaagttt tttaataggg gcaaatagga aaatggagga 9301 taggagtcat ctggggttta tgcagcaaaa ctacaggtat attgcttgta tccgcctcgg 9361 agatttccat gaggagataa agacatgtca cccgagttta tactctcctg cttagatcct 9421 actacagtat gaaatacagt gtygcgaggt agactatgta agcagattta atcattttaa 9481 agagcccagt acttcatatc catttctccc gctccttctg cagccttatc aaaaggtatt 9541 tagaacactc attttagccc cattttcatt tattatactg gcttatccaa cccctagaca 9601 gagcattggc attttccctt tcctgatctt agaagtctga tgactcatga aaccagacag 9661 attagttaca tacaccacaa atcgaggctg tagctggggc ctcaacactg cagttctttt 9721 ataactcctt agtacacttt ttgttgatcc tttgccttga tccttaattt tcagtgtcta 9781 tcacctctcc cgtcaggtgg tgttccacat ttgggcctat tctcagtcca gggagtttta 9841 caacaataga tgtattgaga atccaaccta aagcttaact ttccactccc atgaatgcct 9901 ctctcctttt tctccattat aactgagcta twaccattaa tggtttcagg tggatgtctc 9961 ctcccccaat atacctgatg tatctacata ttgccaggct gatattttaa gacatwaaag 10021 gtatatttca ttattgagcc acatggtatt gattactgct actaaaattt tgtcattgta 10081 cacatctgta aaaggtggtt ccttttggaa tgcaaagttc aggtgtttgt tgtctttcct 10141 gacctaaggt cttgtgagct tgtatttttt ctatttaagc agtgctttct cttggactgg 10201 cttgactcat ggcattctac acgttattgc tggtctaaat gtgattttgc caagcttctt 10261 caggacctat aattttgctt gacttgtagc caaacacaag taaaatgatt aagcaacaaa 10321 tgtatttgtg aagcttggtt tttaggttgt tgtgttgtgt gtgcttgtgc tctataataa 10381 tactatccag gggctggaga ggtggctcgg agttcaagag cacagactgc tcttccagaa 10441 gtcctgagtt caattcccag caaccacatg gtggctcaca accatctgta atgggatctg 10501 atgccctctt ctggtgtgtc tgaagaccac aagtgtattc acattaaata aataatcctc 10561 cttcttcttc tttttttttt tttaaagaga atwctgtctc cagtagaatt actgaagtaa 10621 tgaaatactt tgtgtttgtt ccaatatggw agccaataat caaatactct twagcactgg 10681 aaatgtacca aggaactatt ttatttaagt gwactgtgga cagaggagcc ataactgcag 10741 acttgtggga tacagaagac caatgcagac ttaatgtctt ttctcttaca ctaagcaata 10801 aagaaataaa aattgaactt ctagtatcct atttgttaaa ctgctagctt tactaacttt 10861 tgtgcttcat ctatacaaag ctgaaagcta agtctgcagc cattactaaa catgaaagca 10921 agtaatgata attttggatt tcaaaaatgt agggccagag tttagccagc cagtggtggt 10981 gcttgccttt atgccttaat cccagcactc tggaggcaga gacaggcaga tctctgagtt 11041 tgagcccagc ctggtctaca catcaagttc tatctaggat agccaggaat acacacagaa 11101 accctgttgg ggaggggggc tctgagattt cataaaatta taattgaagc attccctaat 11161 gagccactat ggatgtggct aaatccgtct acctttctga tgagatttgg gtattatttt 11221 ttctgtctct gctgttggtt gggtcttttg acactgtggg ctttcttaaa gcctccttcc 11281 ctgccatgtg gtctcttgtt tgctactaac ttcccatggc ttaaatggca tggctttttg 11341 ccttctaagg gcagctgctg agwtttgcag cctgatttcc agggtggggt tgggaaatct 11401 ttcaaacact aaaattgtcc tttaattttt ttttaaaaaa tgggttatat aataaacctc 11461 ataaaatagt tatgaggagt gaggtggact aatattaatg agtccctccc ctataaaaga 11521 gctattaagg ctttttgtct tatactaact ttttttttaa atgtggtatc tttagaacca 11581 agggtcttag agttttagta tacagaaact gttgcatcgc ttaatcagat tttctagttt 11641 caaatccaga gaatccaaat tcttcacagc caaagtcaaa ttaagaattt ctgactttaa 11701 tgttatttgc tactgtgaat ataaaatgat agcttttcct gaggcagggt ctcactatgt 11761 atctctgcct gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg 11821 aatactaagg ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg 11881 ggacacacta agggagcttg ggtgatagtt ggtaaatgtg tttaagtgat gaaaacttga 11941 attattatca ccgcaaccta ctttttaaaa aaaaaagcca ggcctgttag agcatgctaa 12001 gggatcccta ggacttgctg agcacacaag agtagtactt ggcaggctcc tggtgagagc 12061 atatttcaaa aaacaaggca gacaaccaag aaactacagt aaggttacct gtctttaacc 12121 atctgcatat acacagggat attaaaatat tccaaataat atttcattca agttttcccc 12181 catcaaattg ggacatggat ttctccggtg aataggcaga gttggaaact aaacaaatgt 12241 tggttttgtg atttgtgaaa ttgttttcaa gtgatagtta aagcccatga gatacagaac 12301 aaagctgcta tttcgaggtc tcttggttat actcagaagc acttctttgg gtttccctgc 12361 actatcctga tcatgtgcta ggcctwcctt aggctgattg ttgttcaaat aacttaagtt 12421 tcctgtcagg tgatgtcata tgatttcata tatcaaggca aaacatgtta tatatgttaa 12481 acatttgkac ttaatgtgaa agttaggtct ttgtgggttt tgattttaat ttcaaaacct 12541 gagctaaata agtcatttta catgtcttac atttggtgaa ttgtatattg tggtttgcag 12601 gcaagactct ctgacctagt aaccctccta tagagcactt tgctgggtca caagtctagg 12661 agtcaagcat ttcaccttga agttgagacg ttttgttagt gtatactagt tatatgttgg 12721 aggacatgtt tatccagaag atattcagga ctatttttga ctgggctaag gaattgattc 12781 tgattagcac tgttagtgag cattgagtgg cctttaggct tgaattggag tcacttgtat 12841 atctcaaata atgctggcct tttttwaaaa gcccttgttc tttatcaccc tgttttctac 12901 ataatttttg ttcaaagaaa tacttgtttg gatctccttt tgacaacaat agcatgtttt 12961 caagccatat tttttttcct tttttttttt ttttttggtt tttcgagaca gggtttctct 13021 gtatagccct ggctgtcctg gaactcactt tgtagaccag gctggcctcg aactcagaaa 13081 tccgcctgcc tctgcctcct gagtgccggg attaaaggcg tgcaccacca cgcctggcta 13141 agttggatat tttgtatata actataacca atactaactc cactgggtgg atttttaatt 13201 cagtcagtag tcttaagtgg tctttattgg cccttattaa aatctactgt tcactctaac 13261 agaggctgtt ggactagtgg sactaagcaa cttcctacgg atatactagc agataagggt 13321 cagggataga aactagtcta gcgttttgta tacctaccag cttatactac cttgttctga 13381 tagaaatatt taggacatct agcttatcga tccgtcgagg gggggcccgg tacccagctt 13441 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc 13501 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 13561 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 13621 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 13681 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 13741 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 13801 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 13861 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 13921 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 13981 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 14041 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 14101 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 14161 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 14221 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 14281 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 14341 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 14401 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 14461 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 14521 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 14581 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 14641 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 14701 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 14761 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 14821 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 14881 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 14941 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 15001 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 15061 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 15121 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 15181 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 15241 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 15301 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 15361 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 15421 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 15481 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 15541 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 15601 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg 15661 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 15721 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 15781 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 15841 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 15901 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 15961 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 16021 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 16081 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 16141 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 16201 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg 16261 taatacgact cactataggg cgaattggag ctcca //