LOCUS Exported 3428 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pKJ1267 (aka pAC-alphaGal4) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3428) TITLE OPEN plasmids REFERENCE 2 (bases 1 to 3428) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3428 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 134..153 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(220..322) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind complement(714..731) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(848..1393) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(928..947) /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 1624..1654 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" protein_bind 1662..1678 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 1667..1689 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 1686..1702 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 1686..1702 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS complement(2988..219) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact 601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa 661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc 721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc 781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa 841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc 901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc 961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc 1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac 1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt 1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt 1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg 1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt 1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc 1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca 1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc 1501 atgtttgaca gcttatcatc gataagcttg catgcatcca tcaaaaaaat attgacaaca 1561 taaaaaactt tgtgttatac ttgtaacgct acatggagat taactcaatc tagctagaga 1621 ggctttacac tttatgcttc cggctcgtat aatgtgtgga attgtgagcg gataacaatt 1681 tcacacagga aacagctatg accatgatta cggattcact ggaactctag accaaagaga 1741 ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg atatcgagca 1801 agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct ttggccatac 1861 tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg cggtgaccga 1921 ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc aggaagatat 1981 cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca aagatgaagt 2041 tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata tcacccacga 2101 cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc cacctgaccg atgagaacgc 2161 gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg cttctacccg 2221 aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg catgctacag 2281 ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc gtaccgacct 2341 ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag aggcgattcg 2401 tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac gtgatgtacg 2461 tcagcctgaa gtgaaagaag agaaaccaga ggcggccgca gaatcaaggc tagaaagact 2521 ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga 2581 ttctttacag gatataaaag cattgttaac aggattattt tgaagatctg catcgcagga 2641 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgcta accgttttta 2701 tcaggctctg ggaggcagaa taaatgatca tatcgtcaat tattacctcc acggggagag 2761 cctgagcaaa ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca 2821 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga 2881 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca 2941 ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc 3001 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca 3061 cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa 3121 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca 3181 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct 3241 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga 3301 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca 3361 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt 3421 gccatacg //