LOCUS Exported 910 bp ds-DNA linear SYN 26-MAY-2021 DEFINITION SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene. ACCESSION . VERSION . KEYWORDS pSBbi-RH SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 910) AUTHORS Kowarz E, Loescher D, Marschalek R TITLE Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines. JOURNAL Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821. PUBMED 25650551 REFERENCE 2 (bases 1 to 910) AUTHORS . TITLE Direct Submission JOURNAL Exported May 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..910 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 79..135 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" ORIGIN 1 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag 61 ctgtacaagg gcagtggagc tactaacttc agcctgctga agcaggctgg tgacgtcgag 121 gagaatcctg gccccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg 181 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt 241 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat 301 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg 361 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca 421 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc 481 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca 541 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct 601 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg 661 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg 721 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt 781 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg 841 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag 901 cttgcaggat //