LOCUS       Exported                 779 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    pSBtet-RB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      59..68
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 actcttttgc cttataccta ctttccgtac cacttcctac cctcgaaagg cctctgaggc
       61 caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga
      121 cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac
      181 catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat
      241 gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt
      301 ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg
      361 tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg
      421 catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt
      481 gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca
      541 gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta
      601 cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag
      661 tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt
      721 cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctca
//
