LOCUS       Exported                 807 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    pSBtet-Hyg
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      57..66
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 tcttttgcct tatacctact ttccgtacca cttcctaccc tcgaaaggcc tctgaggcca
       61 ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
      121 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
      181 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
      241 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
      301 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
      361 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
      421 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
      481 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
      541 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
      601 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
      661 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
      721 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
      781 tggtgccatt tcaccacggc ttcggca
//
