LOCUS       Exported                 570 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  dCas9 with single cysteine residue (M1C) for site-specific labelling
            .
ACCESSION   .
VERSION     .
KEYWORDS    10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 570)
  AUTHORS   O'Connell MR, Oakes BL, Sternberg SH, East-Seletsky A, Kaplan M, 
            Doudna JA
  TITLE     Programmable RNA recognition and cleavage by CRISPR/Cas9.
  JOURNAL   Nature. 2014 Sep 28. doi: 10.1038/nature13769.
  PUBMED    25274302
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      24..71
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     67..85
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccggatatag ttcctccttt cancaaaaaa cccctcaaga cccgtttaga ggccccaagg
       61 ggttatgcta gttattgctc agcggtggca gcagccaact cagcttcctt tcgggctttg
      121 ttagcagccg gatcagcgcg cacgcgtggt accggccggc caccaggtgg cgcgccgcga
      181 tcgcggatcc gttatccact tccaatgtta ttagtcacct cctagctgac tcaaatcaat
      241 gcgtgtttca taaagaccag tgatggattg atggataaga gtggcatcta aaacttcttt
      301 tgtagacgta tatcgtttac gatcaattgt tgtatcaaaa tatttaaaag cagcgggagc
      361 tccaagattc gtcaacgtaa ataaatgaat aatattttct gcttgttcac gtattggttt
      421 gtctctatgt ttgttatatg cactaagaac tttatctaaa ttggcatctg ctaaaataac
      481 acgcttagaa aattcactga tttgctcaat aatctcatct aaataatgct tatgctgctc
      541 cacaaacaat tgtttttgtt cgttatcttc
//
