LOCUS Exported 4407 bp ds-DNA linear SYN 02-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pMJ823 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4407) AUTHORS Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E TITLE A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. JOURNAL Science. 2012 Jun 28. PUBMED 22745249 REFERENCE 2 (bases 1 to 4407) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 2, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4407 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..327 /codon_start=1 /gene="trxA" /product="E. coli thioredoxin" /label=TrxA /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL DANLA" CDS 349..366 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 379..399 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" ORIGIN 1 atgagcgata aaattattca cctgactgac gacagttttg acacggatgt actcaaagcg 61 gacggggcga tcctcgtcga tttctgggca gagtggtgcg gtccgtgcaa aatgatcgcc 121 ccgattctgg atgaaatcgc tgacgaatat cagggcaaac tgaccgttgc aaaactgaac 181 atcgatcaaa accctggcac tgcgccgaaa tatggcatcc gtggtatccc gactctgctg 241 ctgttcaaaa acggtgaagt ggcggcaacc aaagtgggtg cactgtctaa aggtcagttg 301 aaagagttcc tcgacgctaa cctggccgga tctggcagtg gttctggtca tcaccatcac 361 catcactccg cgggtagcga gaatctttat tttcagggcg ccatggaaaa accgtacacg 421 attggactag acataggaac aaactcggtt ggatgggcag tgttaacaga tcaatatgat 481 ttagtgaaga ggaaaatgaa aattgctgga gactctgaga aaaaacagat aaagaaaaat 541 ttctggggag ttagactatt tgatgagggt caaacggcag ctgatagaag aatgactaga 601 acagctagaa ggagaataga gagaagacgc aatcgtattt cgtatttaca agggatattt 661 gcggaagaaa tgtctaaggt ggatgcaaat ttcttttgcc gattaagtga tagcttttat 721 gttgataacg aaaaaagaaa tagtcgtcat cctttttttg cgactataga ggaagaagta 781 gaataccaca aaaactaccc aaccatttat cacctgagag aagaactcgt taattcttca 841 gaaaaagctg atttaagatt agtttattta gctttagctc atattataaa atatcgaggg 901 aactttttaa ttgagggagc attagatacc caaaatactt ctgttgatgg aatatataag 961 caatttatac aaacatataa tcaagtgttt gcaagtggca ttgaagacgg ttcgctaaaa 1021 aaattggaag ataataagga cgtagctaaa attctcgttg aaaaagttac ccgaaaagaa 1081 aaacttgaga gaattttaaa attataccct ggtgaaaaac ctgccggaat gtttgctcag 1141 tttatcagct tgattgtagc cagtaaaggg aattttcaaa agccttttga tcttattgag 1201 aaatcggata ttgaatgtgc gaaagatagc tatgaagaag atttagagtc tttgttagcg 1261 ctaattggag atgaatatgc ggaacttttt gttgcagcga aaaatgcata tagtgctgtg 1321 gtgttatcta gtattatcac agtggctgag gctgaaacaa acgcgaagtt atccgcaagt 1381 atgattgaac gttttgatac acatgaagaa gatttagggg aattgaaagc atttatcaag 1441 ctccatcttc ctaaacacta tgaagaaata tttagtaata ccgaaaaaca tggttacgca 1501 ggttatatag acggtaaaac aaagcaggca gacttctata aatacatgaa aacaacatta 1561 gagaacattg aaggccccga ttattttatt gctaaaatag aaaaagaaaa ctttttacga 1621 aaacaacgaa cctttgataa tggggctatc ccgcatcaat tacatttaga agaattagaa 1681 gctattttac accagcaagc aaaatattat ccttttttaa aagaaaacta tgataaaatc 1741 aaaagtttag taacttttag aattccatat tttgttggtc cgctagctaa cggacagagc 1801 gaattcgcat ggttaacaag aaaagctgat ggtgagatta gaccgtggaa tatagaagag 1861 aaagtggatt tcggtaagtc agcggtcgat ttcattgaaa agatgaccaa taaagataca 1921 tatttaccta aagaaaatgt attgcctaaa cacagtctct gttatcaaaa atatttggtt 1981 tataatgagt taaccaaagt tagatacatt aatgaccaag ggaagactag ctatttttct 2041 gggcaggaaa aagaacagat ttttaatgac ttatttaagc aaaagcgtaa agttaaaaag 2101 aaggatttag agctgttctt acgcaatatg agccatgttg aaagtccaac tattgaaggc 2161 ctggaagact cgtttaattc aagttattct acctatcatg atttactcaa ggtagggata 2221 aaacaagaaa tccttgataa cccagtaaat acggaaatgc tagaaaatat agtcaaaatt 2281 ttaactgtgt ttgaagataa acgtatgatt aaagagcagt tacagcaatt ttctgatgtt 2341 ttagatgggg ttgttttgaa aaaattagaa cgacgacatt atacaggatg gggaaggtta 2401 tcagctaagt tattaatggg aattcgtgat aagcaatctc acttaactat tttagattat 2461 ttgatgaatg atgatgggct taatcgtaat ctgatgcaac ttataaatga cagtaattta 2521 tcttttaaat caataattga aaaagaacaa gtaactacag cagataaaga tattcaaagt 2581 attgtggcag accttgctgg tagcccggct ataaaaaaag gtattttaca aagcttgaaa 2641 attgtcgatg aacttgttag tgtaatgggc tacccacctc aaactattgt tgtggaaatg 2701 gcaagagaaa atcaaacaac aggaaaagga aaaaataatt ctagaccacg ttataaatca 2761 ttagaaaaag cgattaaaga atttggtagt caaattctaa aagagcatcc gactgataat 2821 caagaattaa gaaataatag attgtattta tattatttgc aaaatggtaa ggatatgtat 2881 actggacaag aattagatat tcataatctt tctaattatg atattgatca tattgtgcca 2941 cagagtttta taacagataa ctccgttgat aatcttgtgt taacaagttc ggctgggaat 3001 cgggaaaaag gggatgatgt tccaccacta gagatcgttc gaaagagaaa agtattctgg 3061 gagaagttat accaagggaa tttgatgagt aaacgtaaat ttgattattt aactaaagct 3121 gaacctggtg gtcttacaga accggataaa gcgagattta ttcatcggca attagtggaa 3181 acccgtcaaa taacgaagaa tgtagctaat attttacatc aacgttttaa ttatgaaaaa 3241 gacgatcatg ggaatactat gaaccaagtt cggattgtaa cattaaaatc tgcactggtt 3301 agccaattcc gtaagcaatt tcaattgtat aaggtgcgtg gagttaatga ttatcaccac 3361 gctcatgatg cttatttaaa tggggtagtc gcgaatacgt tattaaaagt atatcctcaa 3421 ttagagccgg aatttgttta cggcgattac catcaatttg attggtttaa agcgaataaa 3481 gctacggcga aaaaacaatt ttatacaaac atcatgttat tctttgcgca aaaagatcga 3541 ataatagatg aaaacggtga aattctttgg gataaaaaat atttggatac tgtcaaaaaa 3601 gtcatgagtt atcgacaaat gaatattgtt aagaaaacag aaattcaaaa aggagaattt 3661 tcaaaagcta caattaagcc caaaggaaat tccagtaaat taattcctag aaaaactaat 3721 tgggacccga tgaaatatgg tggtcttgat agccctaata tggcatatgc agtggttatt 3781 gaatatgcaa aagggaaaaa taaattagtt ttcgagaaaa aaattatccg tgttactatc 3841 atggaacgta aagcatttga aaaagatgag aaagcctttt tagaagaaca aggttatcgt 3901 cagcctaaag tcctcgcgaa attaccgaaa tatacgttat atgaatgtga ggaaggacgt 3961 cggagaatgc tggctagtgc taatgaagct cagaaaggaa atcagcaggt attgccaaac 4021 cacctggtaa cattactaca ccatgcggca aattgtgagg caagtgatgg gaaaagcctt 4081 gattatattg aaagtaatag agaaatgttt gctgaactac tagcgcacgt atcagaattt 4141 gccaaaagat atacgttagc tgaagctaat ctaaacaaaa ttaatcagct ttttgagcaa 4201 aataaagaag gtgacatcaa ggcaattgca cgatcttttg ttgatttaat ggcattcaat 4261 gcgatgggtg ctccggcaag ttttaaattt tttgaaacca ctattgagcg taaaagatat 4321 aacaatctaa aagaactatt aagctccacc ataatctacc aatcaatcac aggactatat 4381 gaatcacgaa aaaggctgga tgactaa //