LOCUS Exported 1061 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Integration-free (episomal) expression of human OCT3/4 and shRNA against p53. ACCESSION . VERSION . KEYWORDS pCXLE-hOCT3/4-shp53-F SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1061) AUTHORS Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S TITLE A more efficient method to generate integration-free human iPS cells. JOURNAL Nat Methods. 2011 Apr 3. ():. PUBMED 21460823 REFERENCE 2 (bases 1 to 1061) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1061 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 201..514 /label=U6 promoter /note="RNA polymerase III promoter for mouse U6 snRNA (Das et al., 1988)" primer_bind 433..453 /label=mU6-F /note="Mouse U6 promoter, forward primer" primer_bind complement(665..682) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(916..935) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 taaggatgtc aactacagtc agagaacccc tttgtgtttg gtcccccccc gtgtcacatg 61 tggaacaggg cccagttggc aagttgtacc aaccaactga agggattaca tgcactgccc 121 cgcgaagaag gggcagagat gtcgtagtca ggtttagttc gtccggggcg gggatcgatc 181 ctctagagtc gacgctagcg gatccgacgc cgccatctct aggcccgcgc cggccccctc 241 gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg catataatat 301 ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt tctttttaat 361 actagctaca ttttacatga taggcttgga tttctataag agatacaaat actaaattat 421 tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt gtgtgttttg 481 agactataaa tatcccttgg agaaaagcct tgtttgggcc gactccagtg gtaatctact 541 tcaagagagt agattaccac tggagtcttt tttggaattc ctgcagcccg ggggatccgc 601 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 661 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 721 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 961 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 1021 cgctttctca tagctcacgc tgtaggtatc tcagttcggt g //