LOCUS       Exported                 864 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    pSBtet-RB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             71..127
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             755..862
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDM"
ORIGIN
        1 gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
       61 gggcagtgga gctactaact tcagcctgct gaagcaggct ggtgacgtcg aggagaatcc
      121 tggccccatg tctagactgg acaagagcaa agtcataaac ggcgctctgg aattactcaa
      181 tggagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
      241 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
      301 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
      361 tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg acggggctaa
      421 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
      481 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
      541 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
      601 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
      661 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
      721 gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga
      781 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
      841 tgacgatttt gaccttgaca tgct
//
