LOCUS       Exported                 887 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    pSBtet-RB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             33..89
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             90..833
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 acggcatgga cgagctgtac aagggcagtg gagctactaa cttcagcctg ctgaagcagg
       61 ctggtgacgt cgaggagaat cctggcccca tgtctagact ggacaagagc aaagtcataa
      121 acggcgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg
      181 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
      241 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
      301 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc
      361 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
      421 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
      481 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
      541 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
      601 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
      661 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg
      721 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
      781 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtccggcg
      841 ctactaactt cagcctgctg aagcaggctg gtgacgtcga ggagaat
//
