LOCUS       Exported                 827 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  neomycin resistance; constitutive promoter driving reverse tet 
            activator.
ACCESSION   .
VERSION     .
KEYWORDS    AAVS1-SA-2A-NEO-CAG-RTTA3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Sim X, Cardenas-Diaz FL, French DL, Gadue P
  TITLE     A Doxycycline-Inducible System for Genetic Correction of iPSC 
            Disease Models.
  JOURNAL   Methods Mol Biol. 2015 Jan 29.
  PUBMED    25630922
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    150..679
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 gcggccgcga attcgccctt agagcagagc caggaacccc tgtagggaag gggcaggaga
       61 gccaggggca tgagatggtg gacgaggaag ggggacaggg aagcctgagc gcctctcctg
      121 ggcttgccaa ggactcaaac ccagaagccc agagcagggc cttagggaag cgggaccctg
      181 ctctgggcgg aggaatatgt cccagatagc actggggact ctttaaggaa agaaggatgg
      241 agaaagagaa agggagtaga ggcggccacg acctggtgaa cacctaggac gcaccattct
      301 cacaaaggga gttttccaca cggacacccc cctcctcacc acagccctgc caggacgggg
      361 ctggctactg gccttatctc acaggtaaaa ctgacgcacg gaggaacaat ataaattggg
      421 gactagaaag gtgaagagcc aaagttagaa ctcaggacca acttattctg attttgtttt
      481 tccaaactgc ttctcctctt gggaagtgta aggaagctgc agcaccagga tcagtgaaac
      541 gcaccagaca gccgcgtcag agcagctcag gttctgggag agggtagcgc agggtggcca
      601 ctgagaaccg ggcaggtcac gcatcccccc cttccctccc accccctgcc aagctctccc
      661 tcccaggatc ctctctggct ccatcgtaag caaaccttag aggttctggc aaggagagag
      721 atggctccag gaaatggggg tgtgtcacca gataaggaat ctgcctaaca ggaggtgggg
      781 gttagaccca atatcaggag actaggaagg aggaggccta aggatgg
//
