LOCUS       Exported                 859 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  encodes zinc finger nuclease specific to AAVS1 locus (right).
ACCESSION   .
VERSION     .
KEYWORDS    PGK-AAVS1ZFNR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Sim X, Cardenas-Diaz FL, French DL, Gadue P
  TITLE     A Doxycycline-Inducible System for Genetic Correction of iPSC 
            Disease Models.
  JOURNAL   Methods Mol Biol. 2015 Jan 29.
  PUBMED    25630922
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      36..45
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             48..68
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcctttcga cctgcaagtc gacccgcgga ccggtgccac catggctcca aagaaaaaga
       61 ggaaagtggg aatccacgga gtccccgccg ctatggcaga acgacctttt cagtgcagaa
      121 tttgtatgag gaacttctct cagagctcca atctggcaag gcacatccgc acccatacag
      181 gagagaaacc ctttgcttgc gacatttgtg gccgcaagtt cgcacgaacc gactatctgg
      241 tggatcacac taaaatccat accggcagtc agaagccctt tcagtgccgg atttgtatga
      301 gaaacttctc atacaatacc cacctgacac gacatatccg gactcacacc ggggagaaac
      361 cttttgcttg cgatatttgt ggacggaagt tcgcacaggg ctataacctg gccgggcata
      421 caaaaatcca cctgagaggc tcacagctgg tgaagagcga actggaggag aagaagagcg
      481 agctgaggca taaactgaag tacgtccctc acgagtatat cgaactgatc gagattgcca
      541 ggaattccac tcaggaccgc attctggaaa tgaaagtgat ggagttcttt atgaaggtct
      601 acggatatcg aggcaaacat ctgggcgggt cccgcaagcc tgatggagct atctacaccg
      661 tgggctctcc aatcgactat ggcgtgatcg tcgatacaaa ggcctacagc ggaggctata
      721 acctgcccat cggccaggct gacgaaatgc agcggtacgt gaaggagaac cagactagaa
      781 ataagcacat taacccaaat gaatggtgga aagtgtatcc ctctagtgtc accgagttca
      841 agtttctgtt cgtgtctgg
//
