LOCUS Exported 8496 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAL119-Flt3L SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8496) AUTHORS Ali S, Curtin JF, Zirger JM, Xiong W, King GD, Barcia C, Liu C, Puntel M, Goverdhana S, Lowenstein PR, Castro MG TITLE Inflammatory and anti-glioma effects of an adenovirus expressing human soluble Fms-like tyrosine kinase 3 ligand (hsFlt3L): treatment with hsFlt3L inhibits intracranial glioma progression. JOURNAL Mol Ther. 2004 Dec . 10(6):1071-84. PUBMED 15564139 REFERENCE 2 (bases 1 to 8496) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8496 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..103 /label=ITR /note="inverted terminal repeat of human adenovirus serotype 5" promoter 577..780 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 731..751 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 777..801 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 878..934 /label=MCS /note="pUC18/19 multiple cloning site" CDS 6048..6239 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" primer_bind complement(6233..6255) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 6341..6481 /label=bom /note="basis of mobility region from pBR322" primer_bind 6396..6415 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(6496..6513) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6667..7255) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6747..6766) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7426..8286) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8049..8068 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8287..8391) /gene="bla" /label=AmpR promoter primer_bind 8459..8477 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 181 gtgtgcgccg gtgtanacng gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 241 taaatttggg cgtaaccnag taanatttgg ccattttcgc gggaaaactg aataagagga 301 agtgaaatct gaataattnt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 421 cgggtcaaag ttggcgtttt attattatag tcagctctag actcnagaag cttgcatgcc 481 tgcaggtcaa ttccctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 601 aatgggcgtg gatagcggtt tgactcacgg ggatttncaa gtctccaccc cattgacgtc 661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 781 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 841 agacaccggg accgatccag cctggggatc ttccctgaag cttgcatgcc tgcaggtcga 901 ctctagagga tccccgggta ccgagctcga attccggggc ccccggccga aatgacagtg 961 ctggcgccag cctggagccc aacaacctat ctcctcctgc tgctgctgct gagctcggga 1021 ctcagtggga cccaggactg ctccttccaa cacagcccca tctcctccga cttcgctgtc 1081 aaaatccgtg agctgtctga ctacctgctt caagattacc cagtcaccgt ggcctccaac 1141 ctgcaggacg aggagctctg cgggggcctc tggcggctgg tcctggcaca gcgctggatg 1201 gagcggctca agactgtcgc tgggtccaag atgcaaggct tgctggagcg cgtgaacacg 1261 gagatacact ttgtcaccaa atgtgccttt cagccccccc ccagctgtct tcgcttcgtc 1321 cagaccaaca tctcccgcct cctgcaggag acctccgagc agctggtggc gctgaagccc 1381 tggatcactc gccagaactt ctcccggtgc ctggagctgc agtgtcagcc cgtagagacg 1441 gtgtttcacc gtgtcagcca ggatggtctc gatctcctga cctcgtgatc tgcccgcctc 1501 ggcctcccaa agtgctagga ttacagatac tcctcaaccc tgccaccccc atggagtccc 1561 cggcccctgg aggccacagc cccgacagcc ccgcagcccc ctctgctcct cctactgctg 1621 ctgcccgtgg gcctcctgct gctggccgct gcctggtgcc tgcactggca gaggacgcgg 1681 cggaggacac cccgccctgg ggagcaggtg ccccccgtcc ccagtcccca ggacctgctg 1741 cttgtggagc actgacctgg ccaaggcctc atcctgcgga gccttaaaca acgcagtgag 1801 acagacatct atcatcccat tttacagggg aggatactga ggcacacaga ggggagtcac 1861 cagccagagg atgcatagcc tggacacaga ggaagttggc tagaggccgg tcccttcctt 1921 gggcccctct cattccctcc ccagaatgga ggcaacgcca gaatccagca ccggccccat 1981 ttacccaact ctgtacaaag cccccggaat tcatcgatga tatcagatcc cacgtcacta 2041 ttgtatactc tatattatac tctatgttat actctgtaat cctactcaat aaacgtgtca 2101 cgcctgtgaa accgtactaa gtctcccgtg tcttcttatc accatcaggt gacatcctcg 2161 cccaggctgt caatcatgcc ggtatcgatt ccagtagcac cggccccacg ctgacaaccc 2221 actcttgcag cgttagcagc gcccctctta acaagccgac ccccaccagc gtcgcggtta 2281 ctaacactcc tctccccgac ctgcagccca agcttctcga gtctagagtg gaaggtgctg 2341 aggtacgatg agacccgcac caggtgcaga ccctgcgagt gtggcggtaa acatattagg 2401 aaccagcctg tgatgctgga tgtgaccgag gagctgaggc ccgatcactt ggtgctggcc 2461 tgcacccgcg ctgagtttgg ctctagcgat gaagatacag attgaggtac tgaaatgtgt 2521 gggcgtggct taagggtggg aaagaatata taaggtgggg gtcttatgta gttttgtatc 2581 tgttttgcag cagccgccgc cgccatgagc accaactcgt ttgatggaag cattgtgagc 2641 tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt gatgggctcc 2701 agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta cgagaccgtg 2761 tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc agccaccgcc 2821 cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc agcttcccgt 2881 tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc tttgacccgg 2941 gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc tgccctgaag 3001 gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc tgtttggatt 3061 tggatcaagc aagtgtcttg ctgtctttat ttaggggttt tgcgcgcgcg gtaggcccgg 3121 gaccagcggt ctcggtcgtt gagggtcctg tgtatttttt ccaggacgtg gtaaaggtga 3181 ctctggatgt tcagatacat gggcataagc ccgtctctgg ggtggaggta gcaccactgc 3241 agagcttcat gctgcggggt ggtgttgtag atgatccagt cgtagcagga gcgctgggcg 3301 tggtgcctaa aaatgtcttt cagtagcaag ctgattgcca ggggcaggcc cttggtgtaa 3361 gtgtttacaa agcggttaag ctgggatggg tgcatacgtg gggatatgag atgcatcttg 3421 gactgtattt ttaggttggc tatgttccca gccatatccc tccggggatt catgttgtgc 3481 agaaccacca gcacagtgta tccggtgcac ttgggaaatt tgtcatgtag cttagaagga 3541 aatgcgtgga agaacttgga gacgcccttg tgacctccaa gattttccat gcattcgtcc 3601 ataatgatgg caatgggccc acgggcggcg gcctgggcga agatatttct gggatcacta 3661 acgtcatagt tgtgttccag gatgagatcg tcataggcca tttttacaaa gcgcgggcgg 3721 agggtgccag actgcggtat aatggttcca tccggcccag gggcgtagtt accctcacag 3781 atttgcattt cccacgcttt gagttcagat ggggggatca tgtctacctg cggggcgatg 3841 aagaaaacgg tttccggggt aggggagatc agctgggaag aaagcaggtt cctgagcagc 3901 tgcgacttac cgcagccggt gggcccgtaa atcacaccta ttaccggctg caactggtag 3961 ttaagagagc tgcagctgcc gtcatccctg agcagggggg ccacttcgtt aagcatgtcc 4021 ctgactcgca tgttttccct gaccaaatcc gccagaaggc gctcgccgcc cagcgatagc 4081 agttcttgca aggaagcaaa gtttttcaac ggtttgagac cgtccgccgt aggcatgctt 4141 ttgagcgttt gaccaagcag ttccaggcgg tcccacagct cggtcacctg ctctacggca 4201 tctcgatcca gcatatctcc tcgtttcgcg ggttggggcg gctttcgctg tacggcagta 4261 gtcggtgctc gtccagacgg gccagggtca tgtctttcca cgggcgcagg gtcctcgtca 4321 gcgtagtctg ggtcacggtg aaggggtgcg ctccgggctg cgcgctggcc agggtgcgct 4381 tgaggctggt cctgctggtg ctgaagcgct gccggtcttc gccctgcgcg tcggccaggt 4441 agcatttgac catggtgtca tagtccagcc cctccgcggc gtggcccttg gcgcgcagct 4501 tgcccttgga ggaggcgccg cacgaggggc agtgcagact tttgagggcg tagagcttgg 4561 gcgcgagaaa taccgattcc ggggagtagg catccgcgcc gcaggccccg cagacggtct 4621 cgcattccac gagccaggtg agctctggcc gttcggggtc aaaaaccagg tttcccccat 4681 gctttttgat gcgtttctta cctctggttt ccatgagccg gtgtccacgc tcggtgacga 4741 aaaggctgtc cgtgtccccg tatacagact tgagaggcct gtcctcgacc gatgcccttg 4801 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca 4861 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc 4921 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta 4981 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc 5041 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg 5101 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc 5161 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga 5221 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc 5281 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc 5341 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc 5401 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag 5461 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca 5521 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc 5581 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc 5641 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca 5701 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc 5761 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct 5821 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag 5881 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca 5941 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg 6001 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg 6061 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga 6121 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg 6181 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 6241 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 6301 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 6361 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 6421 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 6481 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 6541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 6601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 6661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 6721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 7021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 7081 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 7141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 7201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7321 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 7381 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 7441 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 7501 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 7561 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 7621 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 7681 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 7741 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 7801 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 7861 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 7921 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 7981 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 8041 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 8101 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8161 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8221 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 8281 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 8341 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 8401 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 8461 taggcgtatc acgaggccct ttcgtcttca agaatt //