LOCUS       Exported                1053 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Probe for HSPB7 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    pCRII HSPB7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(694..712)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(695..712)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(730..746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(730..746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(743..765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    754..770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(778..808)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    823..844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(961..978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaattgtaat acgactcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc
       61 cgccagtgtg atggatatct gcagaattcg cccttattgg gtttggaaca agtggaaatg
      121 agtaaataat ggcaggattt acatgtttgg gtgaactaaa tgtaatttaa agtactaaag
      181 tactatatct actgtatagc catatatatt ctgatatctt tcaatgtttc aattatattt
      241 tggattaaat gtacttccag aattttggag cccactttgt cctgtaagct gatatgtcat
      301 tagcagagag ctgacgttag cagtgcattc atcttaaagg tcttgaatac atgtgacagg
      361 attaaattcc atccaagtct aatgagcaga aatgagagaa ggtcatggag gaatgacaaa
      421 gcagggctaa gtagctatat tttcaaggtc atttatcaca gtgaaacaga cacagcagga
      481 gggcacaggt ctagattttg atctctgtgc ggaacgtctg tgcatgctcc agtttagctg
      541 tgtttctctg tgcttttata gtcagagtcc catcggcccc taaactggac ttcactgaag
      601 tggggtccac atctaagggc gaattccagc acactggcgg ccgttactag tggatccgag
      661 ctcggtacca agcttgatgc atagcttgag tattctatag tgtcacctaa atagcttggc
      721 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      781 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      841 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      901 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      961 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1021 aaaggcggta atacggttat ccacagaatc agg
//
