LOCUS Exported 10792 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Fubi-ChR2-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10792) AUTHORS Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K TITLE Millisecond-timescale, genetically targeted optical control of neural activity. JOURNAL Nat Neurosci. 2005 Sep . 8(9):1263-8. PUBMED 16116447 REFERENCE 2 (bases 1 to 10792) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10792 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2611..3010 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3659..3678 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" regulatory 3789..3798 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3795..4721 /codon_start=1 /product="H134R mutant of an N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2005)" /label=ChR2(H134R) /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGAV" CDS 4743..5456 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4785..4806) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5046..5065) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5393..5414 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5484..6072 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5537..5557) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5955..5966) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6075..6091) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6076..6092) /label=pBluescriptKS /note="For pBluescript vector" LTR 6597..6777 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6803..6820) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6809..7033 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7079..7507 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7166..7185) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7376..7397 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7516..7536) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7521..7850 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7701..7836 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7763..7782 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7898..7945 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7964..8338 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8468..8589 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8505..8524) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8559..8578 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8638..8654) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8638..8654) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8651..8673) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8662..8678 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8686..8716) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8731..8752 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8869..8886) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9040..9628) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9120..9139) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9799..10659) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10422..10441 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10660..10764) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg 2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc 2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag 2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg 2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg 2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac 2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc 3001 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct 3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag 3121 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg 3181 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg 3241 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg 3301 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact 3361 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt 3421 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat 3481 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag 3541 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag 3601 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg 3661 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag 3721 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa 3781 ggggatccgc caccatggac tatggcggcg ctttgtctgc cgtcggacgc gaacttttgt 3841 tcgttactaa tcctgtggtg gtgaacgggt ccgtcctggt ccctgaggat caatgttact 3901 gtgccggatg gattgaatct cgcggcacga acggcgctca gaccgcgtca aatgtcctgc 3961 agtggcttgc agcaggattc agcattttgc tgctgatgtt ctatgcctac caaacctgga 4021 aatctacatg cggctgggag gagatctatg tgtgcgccat tgaaatggtt aaggtgattc 4081 tcgagttctt ttttgagttt aagaatccct ctatgctcta ccttgccaca ggacaccggg 4141 tgcagtggct gcgctatgca gagtggctgc tcacttgtcc tgtcatcctt atccacctga 4201 gcaacctcac cggcctgagc aacgactaca gcaggagaac catgggactc cttgtctcag 4261 acatcgggac tatcgtgtgg ggggctacca gcgccatggc aaccggctat gttaaagtca 4321 tcttcttttg tcttggattg tgctatggcg cgaacacatt ttttcacgcc gccaaagcat 4381 atatcgaggg ttatcatact gtgccaaagg gtcggtgccg ccaggtcgtg accggcatgg 4441 catggctgtt tttcgtgagc tggggtatgt tcccaattct cttcattttg gggcccgaag 4501 gttttggcgt cctgagcgtc tatggctcca ccgtaggtca cacgattatt gatctgatga 4561 gtaaaaattg ttgggggttg ttgggacact acctgcgcgt cctgatccac gagcacatat 4621 tgattcacgg agatatccgc aaaaccacca aactgaacat cggcggaacg gagatcgagg 4681 tcgagactct cgtcgaagac gaagccgagg ccggagccgt gccagcggca ccggtagtag 4741 cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 4801 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 4861 gcaagctgac cctgaagttc atttgcacca ccggcaagct gcccgtgccc tggcccaccc 4921 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 4981 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 5041 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 5101 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 5161 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 5221 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 5281 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 5341 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 5401 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag 5461 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 5521 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 5581 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 5641 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 5701 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5761 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 5821 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 5881 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 5941 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 6001 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 6061 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa 6121 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg 6181 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg 6241 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 6301 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc 6361 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc 6421 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt 6481 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt 6541 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt 6601 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6661 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6721 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg 6781 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 6841 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 6901 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 6961 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 7021 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 7081 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 7141 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 7201 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 7261 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 7321 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 7381 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 7441 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 7501 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 7561 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 7621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 7681 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 7741 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 7801 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 7861 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 7921 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag 7981 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg 8041 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt 8101 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt 8161 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt 8221 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt 8281 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca 8341 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 8401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 8461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 8521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 8581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 8641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 8701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 8761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 8821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 8881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 8941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 9001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 9061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 9121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 9181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 9241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 9301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 9361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 9421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 9481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 9541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 9601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 9661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 9721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 9781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 9841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 9901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 9961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 10021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 10081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 10141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 10201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 10261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 10321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 10381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 10441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 10501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 10561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 10621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 10681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 10741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac //