LOCUS       Exported                8574 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Luminescent reporter of insulin secretion.
ACCESSION   .
VERSION     .
KEYWORDS    Proinsulin-NanoLuc in pLX304
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Burns SM, Vetere A, Walpita D, Dancik V, Khodier C, Perez J, Clemons
            PA, Wagner BK, Altshuler D
  TITLE     High-throughput luminescent reporter of insulin secretion for 
            discovering regulators of pancreatic Beta-cell function.
  JOURNAL   Cell Metab. 2015 Jan 6;21(1):126-37. doi: 
            10.1016/j.cmet.2014.12.010.
  PUBMED    25565210
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             16..57
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    99..687
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(152..172)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(570..581)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             759..992
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1070..1191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1107..1126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1161..1180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1231..1366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1293..1312
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(1386..1405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1387..1405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1415..1432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1415..1431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1424..1446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1573..2028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1660..1679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1870..1891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2054..2158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2159..3019
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2377..2396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3190..3778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3679..3698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3932..3949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4066..4087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4102..4132
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4140..4156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4145..4167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4164..4180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4164..4180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4199..4219
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4201..4219
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4247..4473
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4474..4654
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4701..4826
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5319..5552
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5737..5781
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5956..6456
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6375..6393
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6468..6863
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6923..7040
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7157..7460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7461..7664
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7615..7635
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7687..7711
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7981..8490
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     protein_bind    complement(8564..14)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
       61 taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa
      121 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
      181 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
      241 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
      301 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
      361 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
      421 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
      481 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
      541 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
      601 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
      661 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
      721 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
      781 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
      841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      961 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     1021 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     1081 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1141 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1201 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     1261 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     1321 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     1381 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     1441 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1501 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1561 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     1621 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     1681 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     1741 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     1801 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     1861 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1921 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1981 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     2041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2161 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2221 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2281 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2341 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2401 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2461 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2521 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2581 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2641 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2701 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2761 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2821 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2881 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2941 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3001 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3841 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3901 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3961 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4021 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     4081 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     4141 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     4201 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     4261 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     4321 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     4381 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     4441 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     4501 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4561 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4621 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     4681 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     4741 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     4801 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     4861 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     4921 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     4981 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     5041 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     5101 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     5161 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     5221 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     5281 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     5341 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     5401 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     5461 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     5521 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     5581 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     5641 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     5701 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     5761 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     5821 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     5881 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     5941 accccgaggg gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     6001 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     6061 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     6121 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     6181 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     6241 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     6301 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     6361 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     6421 tccctcgttg accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt
     6481 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     6541 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     6601 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     6661 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     6721 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     6781 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     6841 cctctggtta tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac
     6901 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     6961 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     7021 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     7081 ggctcgaggg ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7141 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7201 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7261 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7321 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7381 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7441 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7501 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7561 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     7621 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactgtcg
     7681 ggatcaacaa gtttgtacaa aaaagcaggc ttaatggccc tgtggatgcg cttcctgccc
     7741 ctgctggccc tgctcttcct ctgggagtcc caccccaccc aggcttttgt caagcagcac
     7801 ctttgtggtt cccacctggt ggaggctctc tacctggtgt gtggggagcg tggcttcttc
     7861 tacacaccca tgtcccgccg tgaagtggag gacccacaag tggcacaact ggagctgggt
     7921 ggaggcccgg gagcaggtga ccttcagacc ttggcactgg aggtggccca gcagaagagg
     7981 gtcttcacac tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac
     8041 caagtccttg aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact
     8101 ccgatccaaa ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc
     8161 atcccgtatg aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg
     8221 gtgtaccctg tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc
     8281 gacggggtta cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg
     8341 ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac
     8401 gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc
     8461 ggctggcggc tgtgcgaacg cattctggcg aagcgtggca ttgtagatca gtgctgcacc
     8521 agcatctgct ccctctacca gctggagaac tactgcaact agtacccagc tttc
//
