LOCUS       Exported                 820 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Orange fluorescent protein based pH indicator in bacterial 
            expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    pBAD-pHoran4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             78..95
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             99..131
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             135..158
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     587..606
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctttttatcg caactctcta ctgtttctcc atacccgttt tttgggctaa caggaggaat
       61 taaccatggg gggttctcat catcatcatc atcatggtat ggctagcatg actggtggac
      121 agcaaatggg tcgggatctg tacgacgatg acgataagga tccgagctcg agaatggtga
      181 gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcgca
      241 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      301 acgagggctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      361 gggacatcct gtcccctcag ttcacctacg gctccaaggc ctacgtgaag caccccgccg
      421 acatccccga ctacttcaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      481 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      541 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      601 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      661 agggcgagat caagaagagg ctgaagctga aggacggcgg ccactacacc tccgaggtca
      721 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcggcatca
      781 agttggacat cacctcccac aacgaggact acaccatcgt
//
