LOCUS Exported 6911 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequence. ACCESSION . VERSION . KEYWORDS pNeuroD-ires-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6911) AUTHORS Guerrier S, Coutinho-Budd J, Sassa T, Gresset A, Jordan NV, Chen K, Jin WL, Frost A, Polleux F TITLE The F-BAR domain of srGAP2 induces membrane protrusions required for neuronal migration and morphogenesis. JOURNAL Cell. 2009 Sep 4;138(5):990-1004. doi: 10.1016/j.cell.2009.06.047. PUBMED 19737524 REFERENCE 2 (bases 1 to 6911) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6911 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 2216..2268 /label=MCS /note="multiple cloning site" misc_feature 2270..2856 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2437..2454) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2664..2683 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 2857..3576 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2857..3573 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2902..2923) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3163..3182) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3510..3531 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 3699..3820 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3736..3755) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3790..3809 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3827..4282) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3964..3985) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4176..4195 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4309..4413 /gene="bla" /label=AmpR promoter promoter 4415..4772 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4438..4458) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4623..4758 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4685..4704 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4807..5601 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4861..4880) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5471..5490 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5789..5808) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5833..5880 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6209..6797 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6698..6717 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tgagctcgga ggacacttgc tgggagcctt ggtgtttgta actccgactt 61 ggaatcaagg ccacattaga atgcagcaaa gcatgaacat tcaggaaaga gagatcttac 121 ttcaagttcc gggaatatcc aggtgtagct tttcttttca aaactccctg gtccgttagt 181 cagatcccct gagccataac ttccaccgct ccccaataac tctcctgtag catcccctaa 241 aatggagagg tgagagccag aatccgagtt acatttcact gaatgaggat atgccaactc 301 ttcatagaag ggctgtccca ataaaatctg cctctactgt gcttaacctt caagattttt 361 ctgcggatcc aggtatttaa acgcaacacc cagagtaaat gaaaaaaaac aaaaacaaaa 421 caaaaaaaca accccccccc ccaaaaaaaa agacaatgct ccttgcttgc tctgctctca 481 gccagttgac agaacaaaga aaaagaaaaa ctccaggcag tttatctcta gaatcccaga 541 atgattggtg ccatgtttta ggccaccaaa gaactcccac aatgccttgc ttttcttttt 601 agattcgttc cgggtctgga aggttacttt caatatcttg caaatgccca ggatctcctt 661 agaaccctct ccccctcccc tttcccagat gtaccccatt ccagccactc aaccctgaca 721 ctgtttgcaa actcaagggc aattgccttt acataagcaa ttcaagtagg atgcattcat 781 tgcaaagggg ttgaagaggg taagaactta atgaagagtt agaagaggaa ctggaaagag 841 aaaggaggcc aaccattcct cctcctcctc tgtcactttt cttgggctag aaggacggag 901 gatcatccat ctgaaagatt cctccttcag atggagaaaa taaagactcc ttatttaaga 961 atagataagg ctattccaca atagactttg cttgactcaa aacacagagt ccccagaaag 1021 ggaaacagca cattgaatag agttttattg cagcttctct ttgagcttct tctgaccaag 1081 gccccagaga ttgagcacta ggggctcaag gaaggctgtg attgaattat ggtctgtgtc 1141 agagatgaaa ttcccacctc tggtgcgggg ttgtttaatc ttagcattcc ctagatgagc 1201 tagctgaggt cattcattac tccaggagca tctgaaaacc aacggagcca aggtctgctg 1261 gcaaaaacta gctttagaca caaaattcta gggtttagag ttgaagactc ttagtgaagt 1321 ctctggggta ggagcaggtg accgttaggt ttacatttgg agtgttcaag tgtagggtat 1381 ggaagctgac ttgcaaagga ttctagaaga tgctctagtc aggaaggttg agtcaaggct 1441 gtgtttccag atgaggtcgt ttttatatga aataaatgtt tgcctagaga ggtgatggag 1501 attagaagcc agccaccctt tggtggcaat aagcaaagcc tctgctaaga ggtagaagct 1561 aagagatggg cctctccaga gagaacaaag tcaaactgca ccaccggaga ccattgtcaa 1621 gggaaggacc cttcagtgca cctcagtatc tgggattgtg agtggttcac ctctgtctac 1681 ctctctgcgc taaagcagtc ttcaggctag gacatagact cagtgatctt tctttacctt 1741 tccagctcgc ctcccgcaac cccccccccc cccacatgtc ctctgtcttc tgctgccaca 1801 aagggttaat ctctcctgcg ggtaaaaaca ggtccgcgga gtctctaact ggcgacagat 1861 gggccacttt cttctggcca caaaggggcc ggaatggagc gctccgcggc atacaaatag 1921 gcaggtcacg tggttcccgg ctcttggctg gaccgggaag accatatggc gcatgccggg 1981 gaggaaggag caggggcgga ggtactgtgg gggtgagggg agtggtggtg gaagggggcg 2041 ggaggaaagt tctggggagg ggtgaatgag ggcgggagtc gttcagtctg gacgcgtgcg 2101 cgattgcggg gcgcagggac ctgctcgttg ctcagctcac ggccccgccc gcgctcagca 2161 tcagcaactc ggctatataa ccctagcgcc cgcgccaccg ggacacgagg aattcctcga 2221 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccgc ccctctccct 2281 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 2341 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 2401 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 2461 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 2521 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 2581 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 2641 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg 2701 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta 2761 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt 2821 tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggagctg 2881 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 2941 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 3001 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 3061 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 3121 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 3181 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 3241 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 3301 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 3361 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 3421 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 3481 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 3541 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa 3601 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 3661 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 3721 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 3781 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt 3841 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 3901 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 3961 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 4021 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 4081 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 4141 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 4201 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 4261 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 4321 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 4381 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga 4441 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 4501 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 4561 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 4621 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 4681 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 4741 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg 4801 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 4861 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 4921 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 4981 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 5041 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 5101 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 5161 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 5221 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 5281 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg 5341 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 5401 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 5461 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 5521 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 5581 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc 5641 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 5701 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 5761 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa 5821 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc 5881 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt 5941 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag 6001 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat 6061 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 6121 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 6181 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 6241 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 6301 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 6361 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 6421 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 6481 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 6541 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 6601 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 6661 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 6721 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 6781 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 6841 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 6901 ccgccatgca t //