LOCUS Exported 497 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).. ACCESSION . VERSION . KEYWORDS GANLS-DEVD-BNES SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 497) AUTHORS Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE TITLE Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. JOURNAL Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. PUBMED 25622108 REFERENCE 2 (bases 1 to 497) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..497 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 36..55 /label=T7 /note="T7 promoter, forward primer" promoter 36..54 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 79..88 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(230..248) /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc 61 caagctggct agcgtgccgc caccatggcc tcgagaatgg tgagcaagag cgaggaggtc 121 atcaaagagt tcatgcgctt caaggtgcgc ttggagggct ccatgaacgg ccacgagttc 181 gagatcgagg gcgagggcga gggccgccct tacgagggca cccagaccgc caagctgaag 241 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccct gatcatgtac 301 ggctccaaga tgtacgtgaa gcaccccgcc gacgtccccg attacatgaa gctgtccttc 361 cccgagggct tcaagtggga gcgcgtgatg cacttcgagg acggcggtct ggtgaccgcg 421 acacaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc 481 aacttccccc ccgacgg //