LOCUS Exported 907 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.. ACCESSION . VERSION . KEYWORDS GANES-DEVD-BNLS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 907) AUTHORS Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE TITLE Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. JOURNAL Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. PUBMED 25622108 REFERENCE 2 (bases 1 to 907) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..907 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(22..42) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(46..66) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" primer_bind complement(159..182) /label=DsRed1-C /note="DsRed1, forward primer" primer_bind complement(357..376) /label=mCherry-F /note="mCherry, forward primer" primer_bind 626..644 /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 ctttctcttc ttttttggat ctacctttct cttctttttt ggatctacct ttctcttctt 61 ttttggatcg cttcccgccg ttcccttgta ccgctcgtcc atgcccagac ggtggcggcc 121 ctcggagcgc ccgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag 181 cttggtgtcc acacagtaat cgccgggcag ttgcacgggc ttcttggcca tgtagatggt 241 ctcgaactcc accaggtagt ggccgccgtc cttcagcttc agggcctgat agctatggcc 301 cttcagcacg ccgtccttgg ggtacagcat ctcggtggag gcctcccagc ccatggtctt 361 cttctgcatt acggggccgt cgggggggaa gttggtgccg cgaatcttca ccttgcagat 421 cagcgtgccg tcctgcaggg aggagtcctg ggtaacggtc accagaccgc cgtcttcgaa 481 gttcatcacg cgctcccact tgaagccctc ggggaagggc agcttcttgt aatcggggat 541 gtcggcgggg tgcctcacgt acgctttgga gccgtacatg atctgggggg acaggatgtc 601 ccaggcaaag ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc 661 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca tggagccctc 721 catgcgcacc ttgaagcgca tgaactcttt gatggtggct ccatccacct catcgccgga 781 ggcggtaccg ctcccgatat caagacctgc taatttcaag gctaaggtgc tgccggtgcc 841 atgccccagg tggtggcggc cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc 901 gttgtgg //