LOCUS       Exported                1129 bp ds-DNA     linear   SYN 07-JUN-2021
DEFINITION  mRNA synthesis of tol1 transposase codon modified for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    pCS2-Tol1.zf1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        15..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgtctggatc tacgtaatac gactcactat agttctagag gctcgagagg cctctgcagt
       61 cgacgggccc gggatccgat gacccaattt tagaatctca cctttctggc ctttctgcta
      121 gcaaactcat caatcacatc atcataagag atttgctctc ccacagagtg gttgatgctc
      181 accacagcca ggttggtcag tctctcctga gacatggtgc ttctcaggta agacttgatc
      241 agcttcagct tgctgaaaga gcgctcagcc tgagccacag tcacaggcag ggtcagagca
      301 attctcagag cagtccagaa gtttgggtag atttcagaca ggtccttgtc agacatgaaa
      361 gagatcagct ccagcagaga aggggtggtg ctaggcaggt ttggcaggtt cttgatctcc
      421 tgcaccagct ctctgctgtc caggtcccag ttcttctcaa agtgcaggat gtttcccaga
      481 gcctcacact gctcagtcag ctcctcatct cccagagaag ggaagttggt cagcactcca
      541 aatctgttct gcaccacctc cagggtgctg aatctctcag caatggcaga cagagcctca
      601 tccaccacca cattgaagaa ctccacctcc agcttcttca gagcatcaga gaaaggctca
      661 tcatgagact cataagagaa ctggcacttg gtgcttctga ttctcttctg cttcaggatg
      721 gcctccacat tcatctcctt gcacatctcc ttggcagcca tctgagcatt cacaaatcca
      781 ttggctctgt aagactggag agactgctca gtcttcttca gcagagacac agccaggtcc
      841 acatgcatgt ttggagactg catcagcttg ctcacatgct tgatggtgct caggatgtca
      901 tgccacacca cagtgcagat gttgaatctg taagatccca cctcctcaga cagagactga
      961 gcctcagcct tgatcacagg gtccttggtc ttgtctctca cctcaatcag agcctctctc
     1021 acagcagctc cctggtatct cattggctca atgctcttga tcttgctctc ccatctggtc
     1081 tcagtccaag acttcagggt gatgctcacc tgagacttca ggatggccc
//
