LOCUS Exported 9809 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAV zinc finger donor vector for genomic targeting. ACCESSION . VERSION . KEYWORDS AAV-CAGGS-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9809) AUTHORS Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R TITLE Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. JOURNAL Nat Biotechnol. 2009 Aug 13. ():. PUBMED 19680244 REFERENCE 2 (bases 1 to 9809) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9809 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 33..836 /label=HA-L /note="left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" misc_feature 843..868 /label=SA /note="splice acceptor site" CDS 892..945 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 955..1554 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(955..974) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 1451..1471 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(1586..1603) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1592..1816 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" enhancer 1822..2201 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2203..2480 /label=chicken beta-actin promoter intron 2482..3499 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 3423..3445 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 3507..3526 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 3555..3564 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3561..4280 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3561..4277 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3606..3627) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3867..3886) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4214..4235 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(4370..4389) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 4435..4490 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(4489..4508) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(4851..4867) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 4875..4891 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4899..4929) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4944..4965 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 5039..5875 /label=HA-R /note="right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" primer_bind complement(5915..5934) /label=T7 /note="T7 promoter, forward primer" promoter complement(5916..5934) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5941..5958) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5941..5957) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5950..5972) /label=M13/pUC Forward /note="In lacZ gene" CDS 6095..6397 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6276..6295 /label=ccdB-fwd /note="ccdB gene, forward primer" primer_bind complement(6800..6819) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7414..7433 /label=Neo-F /note="Neomycin resistance gene, forward primer" CDS complement(7794..8654) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8417..8436 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8778..9366 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9267..9286 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9520..9537 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9654..9675 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9690..9720 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9728..9744 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9752..9768 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 9789..9807 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc 61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca 121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc 181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc 241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc 301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg 361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc 421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc 481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc 541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta 601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag 661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt 721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt 781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc 841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga 901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc 961 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 1021 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 1081 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 1141 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 1201 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 1261 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 1321 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 1381 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 1441 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 1501 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga 1561 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 1621 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 1681 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 1741 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 1801 gcggtgggct ctatgggtct cgacattgat tattgactag ttattaatag taatcaatta 1861 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 1921 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 1981 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 2041 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 2101 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 2161 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt 2221 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt 2281 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg 2341 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga 2401 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa 2461 agcgaagcgc gcggcgggcg gggagtcgct gcgacgctgc cttcgccccg tgccccgctc 2521 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 2581 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 2641 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg 2701 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc 2761 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt 2821 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa 2881 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt 2941 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 3001 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 3061 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg 3121 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 3181 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 3241 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 3301 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 3361 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 3421 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 3481 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 3541 tggcaaagaa ttccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 3601 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 3661 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 3721 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 3781 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 3841 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 3901 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 3961 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 4021 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 4081 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 4141 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 4201 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 4261 tggacgagct gtacaagtaa acgcgtgaat tcactcctca ggtgcaggct gcctatcaga 4321 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct 4381 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 4441 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 4501 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 4561 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 4621 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 4681 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 4741 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 4801 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 4861 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 4921 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 4981 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcgacagtac taagctttac 5041 tagggacagg attggtgaca gaaaagcccc atccttaggc ctcctccttc ctagtctcct 5101 gatattgggt ctaaccccca cctcctgtta ggcagattcc ttatctggtg acacaccccc 5161 atttcctgga gccatctctc tccttgccag aacctctaag gtttgcttac gatggagcca 5221 gagaggatcc tgggagggag agcttggcag ggggtgggag ggaagggggg gatgcgtgac 5281 ctgcccggtt ctcagtggcc accctgcgct accctctccc agaacctgag ctgctctgac 5341 gcggctgtct ggtgcgtttc actgatcctg gtgctgcagc ttccttacac ttcccaagag 5401 gagaagcagt ttggaaaaac aaaatcagaa taagttggtc ctgagttcta actttggctc 5461 ttcacctttc tagtccccaa tttatattgt tcctccgtgc gtcagtttta cctgtgagat 5521 aaggccagta gccagccccg tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg 5581 aaaactccct ttgtgagaat ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac 5641 tccctttctc tttctccatc cttctttcct taaagagtcc ccagtgctat ctgggacata 5701 ttcctccgcc cagagcaggg tcccgcttcc ctaaggccct gctctgggct tctgggtttg 5761 agtccttggc aagcccagga gaggcgctca ggcttccctg tcccccttcc tcgtccacca 5821 tctcatgccc ctggctctcc tgccccttcc ctacaggggt tcctggctct gctctaaggg 5881 caagggcgaa ttcgcggccg ctaaattcaa ttcgccctat agtgagtcgt attacaattc 5941 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 6001 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 6061 cccttcccaa cagttgcgca gcctatacgt acggcagttt aaggtttaca cctataaaag 6121 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgccggggcg 6181 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact 6241 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag 6301 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat 6361 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggcatga gattatcaaa 6421 aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc 6481 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa 6541 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc 6601 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt 6661 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca 6721 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 6781 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 6841 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 6901 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 6961 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 7021 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 7081 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 7141 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 7201 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 7261 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg catggcgatg 7321 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 7381 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 7441 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 7501 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattatt aacgcttaca 7561 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatcaggt 7621 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 7681 aatatgtatc cgctcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 7741 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 7801 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 7861 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 7921 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 7981 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 8041 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 8101 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 8161 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 8221 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 8281 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 8341 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 8401 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 8461 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 8521 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 8581 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 8641 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 8701 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 8761 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 8821 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 8881 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 8941 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 9001 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 9061 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 9121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 9181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 9241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 9301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 9361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 9421 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 9481 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 9541 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 9601 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 9661 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 9721 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 9781 aagctcagaa ttaaccctca ctaaaggga //