LOCUS       Exported                 616 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    4TVR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 616)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             23..40
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..73
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt
       61 gtatttccag ggcaatcagc catctacatc agctcgtgcc cgtcttattg atcctggctt
      121 tggaatatat aaggtaaatg aattggtgga tgccagagat gtcggccttg gtgcttggtt
      181 tgaagcacac atacatagtg ttactagagc ttctgatgga cagtcacgtg gcaaaactcc
      241 actgaagaat ggaaatataa agcataaatc caaagagaac acaaataaat tggacagtgt
      301 accctctacg tctaattcag actgtgttgc tgctgatgaa gacgttattt accatatcca
      361 gtatgatgaa tacccagaaa gcggtactct agaaatgaat gtcaaggatc ttagaccacg
      421 agctagaacc attttgaaat ggaatgaact aaatgttggt gatgtggtaa tggttaatta
      481 taatgtagaa agtcctggac aaagaggatt ctggtttgat gcagaaatta ccacattgaa
      541 gacaatctca aggaccaaaa aagaacttcg tgtgaaaatt ttcctggggg gttctgaagg
      601 aacattaaat gactgc
//
