LOCUS Exported 4548 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION UAS transgenesis vector. ACCESSION . VERSION . KEYWORDS pT1UciMP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4548) AUTHORS Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, Burgess HA TITLE Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish. JOURNAL Nucleic Acids Res. 2015 Jan 27. pii: gkv035. PUBMED 25628360 REFERENCE 2 (bases 1 to 4548) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4548 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 106..127 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 142..172 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 180..196 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 185..207 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 204..220 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 204..220 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" regulatory 1558..1567 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(2152..2169) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2152..2168) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2161..2183) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(2377..2396) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2496..2518 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(2556..2574) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2642..2746 /gene="bla" /label=AmpR promoter CDS 2747..3607 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2965..2984) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3778..4366 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4267..4286 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4520..4537 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc 241 atggtccctt tagccagtag cggttctagg cacgggccgt ccgggcggtg gcctggggcg 301 gaaaactgaa ggggggcggc accggcggct cagcccttgt aatatattat atgcaccact 361 attggtttac ttatgtcaca gtttgtaagt ttgtaacagc ctgaacctgg atcagcttag 421 gcctccaagg cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg 481 agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg 541 tcctccgggc tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag 601 aggtcggagt actgtcctcc gacgcaaggt cggagtactg tcctccgaca ctagaggtcg 661 gagtactgtc ctccgacgca aggtcggagt actgtcctcc gacactagag gtcggagtac 721 tgtcctccga cgcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca 781 agggtcgact ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc 841 ctccacacga attcgatact aaggatcctt gtttgtgagt atgatgttaa atataagcaa 901 tcgacgtttg ttttgtcgtt tgttaaatct agcaatgttt tatttcaatt aaagtgttaa 961 ccggttcatt taaaggtgta tttctgaatg agctaacgta ttagctttta acgctagccg 1021 gactgtttgg ttttgaggat caataaacca acgttttttt ctgatttatt ttgctttaaa 1081 atataaaggt ataattcgtc gtcgagaatt ttgggttttg ttcgagttta gaaatttggc 1141 ggcgtttttt tgtaccgcga aacagggcgg tggttcggtg agcttctcaa ttcatctgct 1201 tgacaaacgg ttttcggcaa ctgtaaatgc gctatgatgc ttttattctt ttcagcgtgt 1261 aactttgtta aatataattg actttccgac tatcgacgtt cgtttcatct ccgtgttatt 1321 gtttaatggc cattttctgt ggtaaactgt cgccattttg ttttcagtcg tccgtttgtt 1381 ccgcccttac tcacagctgt gtcagcatta gtgatgactg ttctcacctg gcctgtttgc 1441 tttcattatt gatgaagagc tttctagaga attctgaagc tcgtctttgt cctgaattca 1501 tctgtattca tttaaaaaaa aaaattaacc ttaatttttt tttcttctca gcgtaaagcc 1561 accatggaga tctcgagact agtaattaag tctcagccac cgttaactga acatgtcaaa 1621 acctgtggag actgttgaga tttgatgttc tgaaaagata aagcctataa ataaaatgtt 1681 gcccaaattt cctgcctgat gtttttcttt gtctttgcta catggctttg ctgctcggat 1741 cggctcactc tgtgtatgcc acgttcactt tgtactctcc ttctcacggt aggtttatta 1801 tttttagatg tgcagttagt ttctgtgaaa taacacacca cacactgata ttgtctgtgc 1861 attgacttgg tgagtgcaca ttgtttttga tcttgacata tttatatttg attgatcagg 1921 tgaactgtgt gaatctaaag tgctccatac agatgttctg cattgaaaat attctcattt 1981 tattagtgga agtgagtgta tgccagcggc cgcggtaccc tgcagtctag acatttcagt 2041 tgacgaagac aaacaaagtt ctgttgtgac tatggggggg gggggcgcct ggggatggtc 2101 tcgcccgggg agtaattcag ggtagaaccg ccactgcctt tagcgacatt cactggccgt 2161 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 2221 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 2281 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct 2341 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata 2401 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 2461 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 2521 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 2581 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 2641 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2701 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 2761 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 2821 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 2881 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 2941 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 3001 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 3061 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 3121 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 3181 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 3241 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 3301 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 3361 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3421 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3481 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3541 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3601 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 3661 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 3721 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 3781 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 3841 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 3901 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 3961 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 4021 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 4081 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 4141 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 4201 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 4261 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 4321 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 4381 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 4441 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 4501 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaga //