LOCUS Exported 9540 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION human BRN3A cDNA. ACCESSION . VERSION . KEYWORDS Teto BRN3A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9540) AUTHORS Blanchard JW, Eade KT, Szucs A, Lo Sardo V, Tsunemoto RK, Williams D, Sanna PP, Baldwin KK TITLE Selective conversion of fibroblasts into peripheral sensory neurons. JOURNAL Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 Nov 24. PUBMED 25420069 REFERENCE 2 (bases 1 to 9540) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9540 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1..20) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 192..571 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 573..771 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 726..746 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 789..969 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1016..1141 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1634..1867 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2052..2096 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2394..2511 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2599..2869 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2902..2940 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2937..2961 /label=LNCX /note="Human CMV promoter, forward primer" CDS 3346..3372 /codon_start=1 /product="9xHis affinity tag" /label=9xHis /translation="HHHHHHHHH" misc_feature 4230..4818 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4283..4303) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4701..4712) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4821..4837) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4822..4838) /label=pBluescriptKS /note="For pBluescript vector" LTR 5343..5523 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5549..5566) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5555..5779 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5825..6253 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5912..5931) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6122..6143 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6262..6282) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6267..6596 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6447..6582 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6509..6528 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6644..6691 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6710..7084 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7214..7335 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7251..7270) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7305..7324 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7384..7400) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7384..7400) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7397..7419) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7408..7424 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7432..7462) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7477..7498 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7615..7632) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7786..8374) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7866..7885) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8545..9405) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9168..9187 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9406..9510) /gene="bla" /label=AmpR promoter ORIGIN 1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga 61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa 121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag 181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt 241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg 601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg 661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc 721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg 781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc 1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt 1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt 1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg 1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc 1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca 1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct 1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga 1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg 1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa 1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa 1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg 1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc 1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct 1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac 1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga 1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt 1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa 2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga 2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag 2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg 2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg 2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg 2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa 2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca 2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat 2521 tacagggaca gcagagatcc agtttggtta attaaaggcg tatcacgagg ccctttcgtc 2581 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 2641 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 2701 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt 2761 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg 2821 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2881 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct 2941 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 3001 agacaccggg accgatccag cctcctgcag gtctagagga tcgaattcat gatgtccatg 3061 aacagcaagc agcctcactt tgccatgcat cccaccctcc ctgagcacaa gtacccgtcg 3121 ctgcactcca gctccgaggc catccggcgg gcctgcctgc ccacgccgcc gctgcagagc 3181 aacctcttcg ccagcctgga cgagacgctg ctggcgcggg ccgaggcgct ggcggccgtg 3241 gacatcgccg tgtcccaggg caagagccat cctttcaagc cggacgccac gtaccacacg 3301 atgaacagcg tgccgtgcac gtccacttcc acggtgcctc tggcgcacca ccaccaccac 3361 caccaccacc accaggcgct cgaacccggc gatctgctgg accacatctc ctcgccgtcg 3421 ctcgcgctca tggccggcgc gggcggcgcg ggcgcggcgg ccggcggcgg gctcctgggc 3481 ggctccgcgc accctcaccc gcatatgcac agcctgggcc acctgtcgca ccccgcggcg 3541 gcggccgcca tgaacatgcc gtccgggctg ccgcaccccg ggctggtggc ggcggcggcg 3601 caccacggcg cggcagcggc agcggcggcg gcggcggccg ggcaggtggc agcggcatcg 3661 gcggcggcgg ccgtggtggg cgcagcgggc ctggcgtcca tctgcgactc ggacacggac 3721 ccgcgcgagc tcgaggcgtt cgcggagcgc ttcaagcagc ggcgcatcaa gctgggcgtg 3781 acgcaggccg acgtgggctc ggcgctggcc aacctcaaga tcccgggcgt gggctcactc 3841 agccagagca ccatctgcag gttcgagtcg ctcacgctct cgcacaacaa catgatcgcg 3901 ctcaagccca tcctgcaggc gtggctcgag gaggccgagg gcgcccagcg cgagaaaatg 3961 aacaagcctg agctcttcaa cggcggcgag aagaagcgca agcggacttc catcgccgcg 4021 cccgagaagc gctccctcga ggcctacttc gccgtgcagc cccggccctc gtccgagaag 4081 atcgccgcca tcgccgagaa actggacctc aaaaagaacg tggtgcgggt gtggttttgc 4141 aaccagagac agaagcagaa gcggatgaaa ttctctgcca cttactgagc tagcggatcc 4201 gttaacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa 4261 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 4321 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 4381 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 4441 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 4501 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 4561 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 4621 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 4681 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 4741 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 4801 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa 4861 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg 4921 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg 4981 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 5041 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg 5101 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct 5161 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc 5221 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt 5281 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta 5341 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 5401 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 5461 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 5521 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 5581 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 5641 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 5701 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 5761 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 5821 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 5881 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 5941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 6001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 6061 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 6121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 6181 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 6241 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 6301 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 6361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 6421 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 6481 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 6541 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 6601 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc 6661 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt 6721 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac 6781 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga 6841 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc 6901 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac 6961 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg 7021 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga 7081 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 7141 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 7201 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 7261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 7321 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc 7381 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7441 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7501 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7561 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7621 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7681 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7741 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7801 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7861 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7921 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7981 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8041 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8101 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8161 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8221 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8281 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8401 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacac //