LOCUS       Exported                 524 bp ds-DNA     linear   SYN 30-AUG-2021
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. . Encodes nuclear EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    pMTB-Multibow-hG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        296..314
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     296..313
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    369..402
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     protein_bind    410..443
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    452..485
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      499..508
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 tgttcctttt tactgagccc tacgactctg gctgagtgcc acaccgccgg cagccgcaaa
       61 gcggctcaaa ccattgcctt ttatggtaat aatgagagaa tgcagaggga cttcctttgt
      121 ctggcatatc tgaggcgcgc gctgtcacta gcgcccacca gcggtcagac tgtagaatgc
      181 agagcaaaac aggaagttga ctccagatgg tcacatgctt gctgaaacnc ctctccctgg
      241 cagcagtgca attctaacag tttccctttc ttttacagtt cgccatccta ggcctattta
      301 ggtgacacta tagaagagta caagctactt gttctttttg caggatccgc caccatgact
      361 agctcgagat aacttcgtat agtatacctt atacgaagtt atcgagctca taacttcgta
      421 taggatactt tatacgaagt tataactcga gataacttcg tatagcatac attatacgaa
      481 gttataccgg cagatctcgc caccatggtg agtgtgatta aacc
//
