LOCUS Exported 604 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.. ACCESSION . VERSION . KEYWORDS pMDC32B-OsMIR390-B/c SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 604) AUTHORS Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, Carrington JC TITLE Highly Specific Gene Silencing in a Monocot Species by Artificial MicroRNAs Derived From Chimeric MIRNA Precursors. JOURNAL Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835. PUBMED 25809382 REFERENCE 2 (bases 1 to 604) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..604 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(172..196) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" terminator 260..512 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(529..545) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(529..545) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(542..564) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 553..569 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg 61 gaatataaat gtcaggctcc cttatacaca gccagtctgc acctcgacgg tctcacatgg 121 tttgttctta ccacacgacc aattaaatcg agctcaaggg tgggcgcgcc gacccagctt 181 tcttgtacaa agtggttcga taattcttaa ttaactagtt ctagagcggc cgccaccgcg 241 gtggagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa 301 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt 361 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc 421 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt 481 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattc gtaatcatgt catagctgtt 541 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 601 gtgt //