LOCUS Exported 4871 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS SV40 small + Large T in pENTR1A (w611-7) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4871) TITLE shMDC1 plasmids REFERENCE 2 (bases 1 to 4871) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4871 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 103..189 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 281..308 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind 316..339 /label=pENTR-F /note="pENTR vectors, forward primer" protein_bind 358..457 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS join(564..809,1156..3036) /codon_start=1 /product="SV40 (simian virus 40) large T antigen" /label=large T antigen /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT CFKKPPTPPPEPET" intron 810..1155 /label=large T intron /note="SV40 (simian virus 40) large T antigen intron" protein_bind complement(3100..3199) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(3311..3330) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 3322..4131 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(3389..3408) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 4221..4809 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4710..4729 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac 481 tggatccgcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 541 cttttgcaaa aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc 601 taatggacct tctaggtctt gaaaggagtg cctgggggaa tattcctctg atgagaaagg 661 catatttaaa aaaatgcaag gagtttcatc ctgataaagg aggagatgaa gaaaaaatga 721 agaaaatgaa tactctgtac aagaaaatgg aagatggagt aaaatatgct catcaacctg 781 actttggagg cttctgggat gcaactgagg tatttgcttc ttccttaaat cctggtgttg 841 atgcaatgta ctgcaaacaa tggcctgagt gtgcaaagaa aatgtctgct aactgcatat 901 gcttgctgtg cttactgagg atgaagcatg aaaatagaaa attatacagg aaagatccac 961 ttgtgtgggt tgattgctac tgcttcgatt gctttagaat gtggtttgga cttgatcttt 1021 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa 1081 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt 1141 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc 1201 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact 1261 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac 1321 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct 1381 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa 1441 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt 1501 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc 1561 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact 1621 agagatccat tttctgttat tgaggaaagt ttgccaggtg ggttaaagga gcatgatttt 1681 aatccagaag aagcagagga aactaaacaa gtgtcctgga agcttgtaac agagtatgca 1741 atggaaacaa aatgtgatga tgtgttgtta ttgcttggga tgtacttgga atttcagtac 1801 agttttgaaa tgtgtttaaa atgtattaaa aaagaacagc ccagccacta taagtaccat 1861 gaaaagcatt atgcaaatgc tgctatattt gctgacagca aaaaccaaaa aaccatatgc 1921 caacaggctg ttgatactgt tttagctaaa aagcgggttg atagcctaca attaactaga 1981 gaacaaatgt taacaaacag atttaatgat cttttggata ggatggatat aatgtttggt 2041 tctacaggct ctgctgacat agaagaatgg atggctggag ttgcttggct acactgtttg 2101 ttgcccaaaa tggattcagt ggtgtatgac tttttaaaat gcatggtgta caacattcct 2161 aaaaaaagat actggctgtt taaaggacca attgatagtg gtaaaactac attagcagct 2221 gctttgcttg aattatgtgg ggggaaagct ttaaatgtta atttgccctt ggacaggctg 2281 aactttgagc taggagtagc tattgaccag tttttagtag tttttgagga tgtaaagggc 2341 actggagggg agtccagaga tttgccttca ggtcagggaa ttaataacct ggacaattta 2401 agggattatt tggatggcag tgttaaggta aacttagaaa agaaacacct aaataaaaga 2461 actcaaatat ttccccctgg aatagtcacc atgaatgagt acagtgtgcc taaaacactg 2521 caggccagat ttgtaaaaca aatagatttt aggcccaaag attatttaaa gcattgcctg 2581 gaacgcagtg agtttttgtt agaaaagaga ataattcaaa gtggcattgc tttgcttctt 2641 atgttaattt ggtacagacc tgtggctgag tttgctcaaa gtattcagag cagaattgtg 2701 gagtggaaag agagattgga caaagagttt agtttgtcag tgtatcaaaa aatgaagttt 2761 aatgtggcta tgggaattgg agttttagat tggctaagaa acagtgatga tgatgatgaa 2821 gacagccagg aaaatgctga taaaaatgaa gatggtgggg agaagaacat ggaagactca 2881 gggcatgaaa caggcattga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca 2941 cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 3001 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttgg 3061 atccggtacc gaattcgcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag 3121 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc 3181 aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga 3241 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata 3301 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta 3361 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa 3421 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa 3481 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg 3541 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg 3601 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat 3661 tcaggtgaaa atattgttga tgcgctggca gtgtccctgc gccggttgca ttcgattcct 3721 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga 3781 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt 3841 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact 3901 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt 3961 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc 4021 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat 4081 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg 4141 gttaattggt tgtaacatta ttcagattgg gccccgttcc actgagcgtc agacccggta 4201 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 4261 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 4321 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 4381 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 4441 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 4501 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 4561 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 4621 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 4681 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 4741 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 4801 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 4861 gctcacatgt t //