LOCUS       Exported                4999 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCI-OR51E1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Saito H, Chi Q, Zhuang H, Matsunami H, Mainland JD
  TITLE     Odor coding by a Mammalian receptor repertoire.
  JOURNAL   Sci Signal. 2009  . 2(60):ra9.
  PUBMED    19261596
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1739..1753)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    2113..2234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2124..2143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2178..2197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2415..2870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2502..2521)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2712..2733
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2937..2956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3056..3078
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3116..3134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3202..3306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3307..4167
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3525..3544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4338..4926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4827..4846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcatg aatggcacag aaggccctaa
     1081 cttctacgtg cccttctcca atgcgacggg tgtggtacgc gaattcacgc gtatgatggt
     1141 ggatcccaat ggcaatgaat ccagtgctac atacttcatc ctaataggcc tccctggttt
     1201 agaagaggct cagttctggt tggccttccc attgtgctcc ctctacctta ttgctgtgct
     1261 aggtaacttg acaatcatct acattgtgcg gactgagcac agcctgcatg agcccatgta
     1321 tatatttctt tgcatgcttt caggcattga catcctcatc tccacctcat ccatgcccaa
     1381 aatgctggcc atcttctggt tcaattccac taccatccag tttgatgctt gtctgctaca
     1441 gatgtttgcc atccactcct tatctggcat ggaatccaca gtgctgctgg ccatggcttt
     1501 tgaccgctat gtggccatct gtcacccact gcgccatgcc acagtactta cgttgcctcg
     1561 tgtcaccaaa attggtgtgg ctgctgtggt gcggggggct gcactgatgg caccccttcc
     1621 tgtcttcatc aagcagctgc ccttctgccg ctccaatatc ctttcccatt cctactgcct
     1681 acaccaagat gtcatgaagc tggcctgtga tgatatccgg gtcaatgtcg tctatggcct
     1741 tatcgtcatc atctccgcca ttggcctgga ctcacttctc atctccttct catatctgct
     1801 tattcttaag actgtgttgg gcttgacacg tgaagcccag gccaaggcat ttggcacttg
     1861 cgtctctcat gtgtgtgctg tgttcatatt ctatgtacct ttcattggat tgtccatggt
     1921 gcatcgcttt agcaagcggc gtgactctcc gctgcccgtc atcttggcca atatctatct
     1981 gctggttcct cctgtgctca acccaattgt ctatggagtg aagacaaagg agattcgaca
     2041 gcgcatcctt cgacttttcc atgtggccac acacgcttca gagccctagg cggccgcttc
     2101 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2161 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2221 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     2281 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     2341 cgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     2401 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     2461 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     2521 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2581 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     2641 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2701 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     2761 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2821 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg
     2881 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     2941 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     3001 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3061 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     3121 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3181 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3241 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3301 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3361 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3421 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3481 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3541 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3601 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3661 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3721 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3781 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3841 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3901 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3961 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4021 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4081 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4141 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4321 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4381 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4441 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4501 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4561 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4621 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4681 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4741 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4801 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4861 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4921 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4981 cacatggctc gacagatct
//
