LOCUS Exported 9245 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Inducible expression of Ngn1. ACCESSION . VERSION . KEYWORDS Teto Ngn1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9245) AUTHORS Blanchard JW, Eade KT, Szucs A, Lo Sardo V, Tsunemoto RK, Williams D, Sanna PP, Baldwin KK TITLE Selective conversion of fibroblasts into peripheral sensory neurons. JOURNAL Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 Nov 24. PUBMED 25420069 REFERENCE 2 (bases 1 to 9245) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9245 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1..20) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 192..571 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 573..771 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 726..746 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 789..969 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1016..1141 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1634..1867 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2052..2096 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2394..2511 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2599..2869 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2902..2940 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2937..2961 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 3935..4523 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3988..4008) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4406..4417) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4526..4542) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4527..4543) /label=pBluescriptKS /note="For pBluescript vector" LTR 5048..5228 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5254..5271) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5260..5484 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5530..5958 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5617..5636) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5827..5848 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5967..5987) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5972..6301 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6152..6287 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6214..6233 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6349..6396 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6415..6789 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6919..7040 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6956..6975) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7010..7029 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7089..7105) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7089..7105) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7102..7124) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7113..7129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7137..7167) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7182..7203 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7320..7337) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7491..8079) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7571..7590) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8250..9110) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8873..8892 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9111..9215) /gene="bla" /label=AmpR promoter ORIGIN 1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga 61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa 121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag 181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt 241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg 601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg 661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc 721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg 781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc 1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt 1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt 1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg 1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc 1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca 1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct 1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga 1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg 1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa 1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa 1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg 1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc 1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct 1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac 1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga 1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt 1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa 2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga 2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag 2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg 2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg 2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg 2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa 2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca 2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat 2521 tacagggaca gcagagatcc agtttggtta attaaaggcg tatcacgagg ccctttcgtc 2581 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 2641 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 2701 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt 2761 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg 2821 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2881 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct 2941 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 3001 agacaccggg accgatccag cctcctgcag gtctagagcc accatgcctg cccctttgga 3061 gacctgcatc tctgatctcg actgctccag cagcaacagc agcagcgacc tgtccagctt 3121 cctcaccgac gaggaggact gtgccaggct acagccccta gcctccacct cggggctgtc 3181 cgtgccagcc cggaggagcg ctcccgccct ctccggggca tcgaatgttc ccggtgccca 3241 ggacgaagag caggaacggc ggaggcggcg aggtcgcgct cgggtgcggt ccgaggctct 3301 gctgcactcc ctgcggagga gtcgtcgcgt caaagccaac gatcgcgagc gcaaccgcat 3361 gcacaacctc aacgctgcgc tggacgcctt gcgcagcgtg ctgccctcgt tccccgacga 3421 caccaagctc accaagattg agacgctgcg cttcgcctac aactacatct gggccctggc 3481 tgagacactg cgcctggcag atcaagggct ccccgggggc agtgcccggg agcgcctcct 3541 gcctccgcag tgtgtcccct gtctgcccgg gcccccgagc ccggccagcg acactgagtc 3601 ctggggttcc ggggccgctg cctccccctg cgccactgtg gcatcaccac tctctgaccc 3661 cagtagtccc tcggcttcag aagacttcac ctatggcccg ggcgatcccc ttttctcctt 3721 tcctggcctg cccaaagacc tgctccacac gacgccctgt ttcatcccat accactaggc 3781 ctttgtaagg caacatcaat actttcttcc tcccccagtc taagagcaat aatagatggg 3841 gaactggctg aagcctccgg gggccacact tacccccaag tgaattcttc gaagctagcg 3901 gatccgttaa caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg 3961 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4021 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4081 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4141 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 4201 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 4261 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 4321 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 4381 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 4441 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 4501 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg 4561 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca 4621 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta 4681 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 4741 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt 4801 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg 4861 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa 4921 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt 4981 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga 5041 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5101 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5161 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5221 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 5281 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 5341 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 5401 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 5461 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 5521 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 5581 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 5641 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 5701 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 5761 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 5821 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 5881 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 5941 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 6001 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 6061 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 6121 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 6181 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 6241 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 6301 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa 6361 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc 6421 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc 6481 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc 6541 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc 6601 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg 6661 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg 6721 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag 6781 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc 6841 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 6901 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 6961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 7021 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 7081 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 7141 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 7201 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 7261 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 7321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 7381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 7441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 7501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 7561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 7621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 7681 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 7741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 7801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 7861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 7921 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 7981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 8041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 8101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 8161 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 8221 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 8281 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 8341 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 8401 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 8461 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 8521 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 8581 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 8641 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 8701 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 8761 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 8821 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 8881 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 8941 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 9001 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 9061 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 9121 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 9181 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 9241 gacac //