LOCUS Exported 1997 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MXS_chaining vector with MCS1. ACCESSION . VERSION . KEYWORDS MXS_MCS1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1997) AUTHORS Sladitschek HL, Neveu PA TITLE MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. JOURNAL PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PUBMED 25909630 REFERENCE 2 (bases 1 to 1997) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1997 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 105..209 /gene="bla" /label=AmpR promoter CDS 210..1070 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(428..447) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1241..1829 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1730..1749 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgatatca aacgcgtaac tcgaggctag ctatatctag atataccatg gtataggtac 61 ctatatgatc atataggatc cgtcgaccaa ttcggggaaa tgtgcgcgga acccctattt 121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 421 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 721 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 1021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 1381 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 1981 gcagcgagtc agtgagc //