LOCUS Exported 1946 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MXS_chaining vector with MCS4. ACCESSION . VERSION . KEYWORDS MXS_MCS4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1946) AUTHORS Sladitschek HL, Neveu PA TITLE MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. JOURNAL PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PUBMED 25909630 REFERENCE 2 (bases 1 to 1946) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1946 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 54..158 /gene="bla" /label=AmpR promoter CDS 159..1019 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(377..396) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1190..1778 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1679..1698 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgacgcgt aactcgaggc agctgagatc tgtcgacaat tcggggaaat gtgcgcggaa 61 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 121 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 181 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 241 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 301 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 361 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 421 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 481 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 541 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 601 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 661 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 721 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 781 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 841 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 901 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 961 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 1021 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 1081 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 1141 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 1201 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 1261 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 1321 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 1381 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 1441 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 1501 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 1561 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 1621 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 1681 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 1741 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 1801 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 1861 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 1921 cgaccgagcg cagcgagtca gtgagc //