LOCUS Exported 2658 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MXS_chaining vector with mEGFP. ACCESSION . VERSION . KEYWORDS MXS_mEGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2658) AUTHORS Sladitschek HL, Neveu PA TITLE MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. JOURNAL PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PUBMED 25909630 REFERENCE 2 (bases 1 to 2658) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2658 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 26..742 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(71..92) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(332..351) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 679..700 /label=EGFP-C /note="EGFP, forward primer" promoter 766..870 /gene="bla" /label=AmpR promoter CDS 871..1731 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1089..1108) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1902..2490 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2391..2410 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgatatca aacgcgtaac tcgagatggt gagcaagggc gaggagctgt tcaccggggt 61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 181 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 241 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 481 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc 661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 721 cggcatggac gagctgtaca aggtcgacca attcggggaa atgtgcgcgg aacccctatt 781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 841 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 901 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 961 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 1021 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 1081 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 1141 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 1201 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 1261 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 1321 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 1381 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 1441 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 1501 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 1561 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 1621 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 1681 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 1741 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 1801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 1861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 1921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 1981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 2161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 2221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 2281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 2341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 2401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 2461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 2521 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 2581 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 2641 cgcagcgagt cagtgagc //