LOCUS Exported 3115 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MXS_chaining vector with EF1a. ACCESSION . VERSION . KEYWORDS MXS_EF1a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3115) AUTHORS Sladitschek HL, Neveu PA TITLE MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. JOURNAL PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PUBMED 25909630 REFERENCE 2 (bases 1 to 3115) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3115 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 30..1192 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 261..1183 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1140..1160 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" promoter 1223..1327 /gene="bla" /label=AmpR promoter CDS 1328..2188 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1546..1565) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2359..2947 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2848..2867 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgatatca aacgcgtaac tcgaggtgag gctccggtgc ccgtcagtgg gcagagcgca 61 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 121 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 181 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 241 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 301 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctccagtac gtgattcttg 361 atcccgagct ggagccaggg gcgggccttg cgctttagga gccccttcgc ctcgtgcttg 421 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc 481 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac 541 gctttttttc tggcaagata gtcttgtaaa tgcgggccag gatctgcaca ctggtatttc 601 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag 661 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc 721 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc 781 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctccaggggg 841 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa 901 aggggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc 961 caggcacctc gattagttct ggagcttttg gagtacgtcg tctttaggtt ggggggaggg 1021 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg 1081 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct 1141 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaacacgctg 1201 tcgaccaatt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 1261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 1321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 1381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 1441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 1501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 1561 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 1621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 1681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 1741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 1801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 1861 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 1921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 1981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 2041 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 2101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 2161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 2221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 2281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 2341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 2401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 2461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 2521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 2581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 2641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 2701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 2761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 2821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 2881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 2941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 3001 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 3061 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagc //