LOCUS Exported 1977 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MXS_chaining vector with linker. ACCESSION . VERSION . KEYWORDS MXS_linker SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1977) AUTHORS Sladitschek HL, Neveu PA TITLE MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. JOURNAL PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PUBMED 25909630 REFERENCE 2 (bases 1 to 1977) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1977 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 85..189 /gene="bla" /label=AmpR promoter CDS 190..1050 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(408..427) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1221..1809 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1710..1729 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgatatca aacgcgtaac tcgagggcca cggcaccggc agcaccggca gcggcagctc 61 cgtcgaccaa ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 181 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 241 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 301 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 361 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 421 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 481 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 541 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 601 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 661 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 721 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 781 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 841 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 901 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 961 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 1021 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 1081 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 1141 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 1201 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 1261 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 1321 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 1381 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 1441 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 1501 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 1561 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 1621 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 1681 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 1741 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 1801 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 1861 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 1921 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagc //