LOCUS Exported 3818 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MXS_chaining vector with CMV::HygroR-bGHpA . ACCESSION . VERSION . KEYWORDS MXS_CMV::HygroR-bGHpA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3818) AUTHORS Sladitschek HL, Neveu PA TITLE MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. JOURNAL PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PUBMED 25909630 REFERENCE 2 (bases 1 to 3818) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3818 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 86..389 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 390..593 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 544..564 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 647..1672 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" polyA_signal 1679..1903 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 1926..2030 /gene="bla" /label=AmpR promoter CDS 2031..2891 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2249..2268) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3062..3650 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3551..3570 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgatatca aacgcgtaac tcgagtagtt attaatagta atcaattacg gggtcattag 61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag 601 tgaaccgtca gatccgctag ggatctcagc tggtcgagtc gccaccatga aaaagcctga 661 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct 721 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg 781 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg 841 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga 901 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga 961 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc 1021 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac 1081 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt 1141 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc 1201 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct 1261 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc 1321 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca 1381 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta 1441 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga 1501 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg 1561 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact 1621 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat aggtcgagct 1681 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 1741 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 1801 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 1861 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggtcgacca attcggggaa 1921 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 1981 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 2041 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 2101 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 2161 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 2221 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 2281 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 2341 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 2401 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 2461 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 2521 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 2581 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 2641 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 2701 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 2761 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 2821 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 2881 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 2941 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 3001 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 3061 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 3121 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 3181 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 3241 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3301 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3361 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3421 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 3481 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 3541 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 3601 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 3661 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 3721 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 3781 gccgcagccg aacgaccgag cgcagcgagt cagtgagc //