LOCUS       Exported                 934 bp ds-DNA     linear   SYN 27-MAY-2021
DEFINITION  Constitutive expression vector for Cre-recombinase containing TRP1 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    pPL5608_TEF1*-Cre_TRP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 934)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(22..40)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    81..584
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        621..725
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
       61 taataatggt ttcttagacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta
      121 atgatggaat aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga
      181 ttttagaaag taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa
      241 aggtttaaaa aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag
      301 cagattaaat agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc
      361 gtgtgtggtc ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga
      421 agagcaagat aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga
      481 aaacaaaaac tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt
      541 tatattaaaa aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg
      601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      901 gccccgaaga acgttttcca atgatgagca cttt
//
