LOCUS       Exported                 679 bp ds-DNA     linear   SYN 17-JUL-2021
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes cytoplasmic dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    pMTB-Multibow-R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        294..312
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     294..311
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    367..400
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     protein_bind    408..441
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    450..483
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      497..506
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 ttccttttta ctgagcccta cgactctggc tgagtgccac accgccggca gccgcaaagc
       61 ggctcaaacc attgcctttt atggtaataa tgagagaatg cagagggact tcctttgtct
      121 ggcatatctg aggcgcgcgc tgtcactagc gcccaccagc ggtcagactg tagaatgcag
      181 agcaaaacag gaagttgact ccagatggtc acatgcttgc tgaaacncct ctccctggca
      241 gcagtgcaat tctaacagtt tccctttctt ttacagttcg ccatcctagg cctatttagg
      301 tgacactata gaagagtaca agctacttgt tctttttgca ggatccgcca ccatgactag
      361 ctcgagataa cttcgtatag tataccttat acgaagttat cgagctcata acttcgtata
      421 ggatacttta tacgaagtta taactcgaga taacttcgta tagcatacat tatacgaagt
      481 tataccggca gatctcgcca ccatggtgag tgtgattaaa ccagagatga agatgaagta
      541 cttcatggac ggctccgtca atgggcatga gttcacagtt gaaggtgaag gcacaggcaa
      601 accttacgag ggacatcaag agatgacact acgcgtcaca atggccaagg gcgggccaat
      661 gcctttctcg tttgactta
//
