LOCUS       Exported                 649 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  An empty sgRNA expression vector for expression of sgRNA for Sp Cas9
            (3rd generation lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    pLenti SpBsmBI sgRNA Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Pham H, Kearns NA, Maehr R
  TITLE     Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian 
            Cells.
  JOURNAL   Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3.
  PUBMED    26463376
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_RNA        complement(103..178)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(206..446)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     complement(256..275)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(426..446)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    477..593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
       61 agtgaacgga tctcgacggt atcggttaac actagaaaaa aagcaccgac tcggtgccac
      121 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacga
      181 gacgcattaa tcgtctcggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg
      241 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact
      301 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg
      361 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat
      421 gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggtaccgga tccaactttt
      481 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      541 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgatg
      601 cctccccgtc accacccccc ccaacccgcc ccgaccggag ctgagagta
//
