LOCUS       Exported                 722 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  An empty sgRNA expression vector for expression of sgRNA for Sp Cas9
            (3rd generation lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    pLenti SpBsmBI sgRNA Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Pham H, Kearns NA, Maehr R
  TITLE     Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian 
            Cells.
  JOURNAL   Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3.
  PUBMED    26463376
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(662..682)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ccatgaccga gtacaagcct actgttcggc tcgcgactcg cgatgatgtc cccagagcag
       61 tgagaacact ggctgctgcc tttgccgatt accccgcaac ccgccatacc gtggatcccg
      121 accggcacat cgagagggtg actgaactgc aggaattgtt tttgacaagg gtgggactcg
      181 acattggaaa ggtgtgggtg gccgatgacg gagccgccgt cgccgtttgg accacaccag
      241 agagcgtcga agccggcgcg gtgttcgccg agatcggccc aagaatggcc gagctctctg
      301 gatctagact ggccgcgcaa cagcagatgg aaggcctgct cgcgccacac cgacctaagg
      361 agcccgcatg gttcctggcc accgtaggcg tttcccccga tcaccaggga aaggggcttg
      421 gcagcgccgt agttctgccc ggcgtggagg ccgctgaacg agctggtgtt cccgcatttc
      481 ttgagaccag tgcacctagg aaccttcctt tctatgaaag gttgggtttc accgtcaccg
      541 ccgatgtgga agtgcctgag ggacctcgaa cctggtgcat gactcgaaag cctggcgcct
      601 aactcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
      661 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
      721 ct
//
