LOCUS Exported 812 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker . ACCESSION . VERSION . KEYWORDS pDRGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 812) AUTHORS Pierce AJ, Johnson RD, Thompson LH, Jasin M TITLE XRCC3 promotes homology-directed repair of DNA damage in mammalian cells. JOURNAL Genes Dev. 1999 Oct 15. 13(20):2633-8. PUBMED 10541549 REFERENCE 2 (bases 1 to 812) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..812 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 31..51 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 73..93 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" regulatory 307..316 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(358..379) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(619..638) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 agatccgccg ccactatggg atcaagatcg ccaaaaaaga agagaaaggt gccgaagaag 61 catgcagcac caccaaaaaa aaaacgaaaa gtagaagacc cacgaggcaa caccagcggc 121 gtgctgagca cccccaaggc caagagggcc aagcaccccc ccggcaccga gaagcccagg 181 agcaggagcc agagcgagca gcccgccacc tgccccatct gctacgccgt gatcaggcag 241 agcaggaacc tgaggaggca cctggagctg aggcacttcg ccaagcccgg cgtggatcca 301 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 361 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcta gggataacag 421 ggtaatacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 481 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 541 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 601 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 661 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 721 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 781 aagcagaaga acggcatcaa ggtgaacttc aa //