LOCUS Exported 800 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker . ACCESSION . VERSION . KEYWORDS pDRGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 800) AUTHORS Pierce AJ, Johnson RD, Thompson LH, Jasin M TITLE XRCC3 promotes homology-directed repair of DNA damage in mammalian cells. JOURNAL Genes Dev. 1999 Oct 15. 13(20):2633-8. PUBMED 10541549 REFERENCE 2 (bases 1 to 800) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..800 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 170..189 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 429..450 /label=EGFP-N /note="EGFP, reverse primer" regulatory 492..501 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS complement(715..735) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(757..777) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 agttcacctg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta 61 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag 121 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt 181 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt 241 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt 301 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt 361 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg 421 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc 481 gcccttgctc accatggtgg cgaccggtgg atccacgccg ggcttggcga agtgcctcag 541 ctccaggtgc ctcctcaggt tcctgctctg cctgatcacg gcgtagcaga tggggcaggt 601 ggcgggctgc tcgctctggc tcctgctcct gggcttctcg gtgccggggg ggtgcttggc 661 cctcttggcc ttgggggtgc tcagcacgcc gctggtgttg cctcgtgggt cttctacttt 721 tcgttttttt tttggtggtg ctgcatgctt cttcggcacc tttctcttct tttttggcga 781 tcttgatccc taaagcttgg //